Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free (AB250910)

This data was developed using ab187147, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue, labeling PGAM2 with ab187147 at 1/4000 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free (AB250910)

This data was developed using the same antibody clone in a different buffer formulation (abAB187147).

Western blot : Anti-PGAM antibody [EPR15194(B)] (ab187147) staining at 1/10000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab187147 was shown to bind specifically to PGAM. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PGAM2 antibody [EPR15194(B)] (<a href='/en-us/products/primary-antibodies/pgam2-antibody-epr15194b-ab187147'>ab187147</a>) at 1/10000 dilution

Lane 1:

Recombinant human PGAM2 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam2-protein-active-ab219276'>ab219276</a> cell lysate at 0.1 µg

Lane 2:

Recombinant human PGAM1 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam1-protein-active-ab289791'>ab289791</a> cell lysate at 0.1 µg

Lane 3:

PGAM4 Human Recombinant Protein cell lysate at 0.1 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

A431 cell lysate at 20 µg

Lane 6:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free (AB250910)

This data was developed using ab187147, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGAM2 antibody [EPR15194(B)] (<a href='/en-us/products/primary-antibodies/pgam2-antibody-epr15194b-ab187147'>ab187147</a>) at 1/10000 dilution

Lane 1:

Rat skeletal muscle tissue lysate at 10 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 25 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free (AB250910)

This data was developed using ab187147, the same antibody clone in a different buffer formulation.

Western blot analysis of PGAM2 in immunoprecipitation pellets from Human skeletal muscle lysate. ab187147 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-PGAM2 antibody [EPR15194(B)] (<a href='/en-us/products/primary-antibodies/pgam2-antibody-epr15194b-ab187147'>ab187147</a>)

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-PGAM2 antibody [EPR15194(B)] - BSA and Azide free (AB250910)

This data was developed using ab187147, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGAM2 antibody [EPR15194(B)] (<a href='/en-us/products/primary-antibodies/pgam2-antibody-epr15194b-ab187147'>ab187147</a>) at 1/10000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 25 kDa

false