Anti-PGAM4 + PGAM1 antibody [OTI2A12]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (AB279384)

Paraffin embedded human embryonic cerebellum stained for PGAM4 + PGAM1 using ab279384 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (AB279384)

Paraffin embedded human adult heart tissue stained for PGAM4 + PGAM1 using ab279384 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

• WB

Supplier Data

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (AB279384)

All lanes:

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (ab279384) at 1/500 dilution

Lane 1:

HEK-293T cell extract at 35 µg

Lane 2:

MCF7 cell extract at 35 µg

Predicted band size: 29 kDa

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• WB

Lab

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (AB279384)

Western blot : Anti-PGAM antibody [OTI2A12] (ab279384) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab279384 was shown to bind specifically to PGAM. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (ab279384) at 1/1000 dilution

Lane 1:

Recombinant human PGAM2 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam2-protein-active-ab219276'>ab219276</a> cell lysate at 0.1 µg

Lane 2:

Recombinant human PGAM1 protein (Active) <a href='/en-us/products/proteins-peptides/recombinant-human-pgam1-protein-active-ab289791'>ab289791</a> cell lysate at 0.1 µg

Lane 3:

PGAM4 Human Recombinant Protein cell lysate at 0.1 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

A431 cell lysate at 20 µg

Lane 6:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 29 kDa

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• WB

Supplier Data

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (AB279384)

All lanes:

Western blot - Anti-PGAM4 + PGAM1 antibody [OTI2A12] (ab279384) at 1/2000 dilution

Lane 1:

HEK-293T cells transfected with the pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected pCMV6-ENTRY PGAM4 cDNA for 48 hrs and lysed at 5 µg

Predicted band size: 29 kDa

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