Anti-PGAM5 antibody [CL0624] (ab244218)

Mouse Monoclonal PGAM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGAM5 aa 50-150.

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Images

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human PGAM5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96HS1

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Predicted	Predicted
Rat	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information PGAM5

Function

Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed:24746696, PubMed:32439975). Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1 (PubMed:32439975). Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation (PubMed:37498743). Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3 (PubMed:24746696). Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2 (PubMed:18387606). Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria (PubMed:22265414).

Alternative names

Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, PGAM5

Recommended products

Mouse Monoclonal PGAM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGAM5 aa 50-150.

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human PGAM5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q96HS1
Clone number: CL0624
Purification technique: Affinity purification Protein A
Epitope: Binds to an epitope located within the peptidesequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PGAM5 also known as Phosphoglycerate Mutase Family Member 5 is a mitochondrial protein with a known mass of around 32 kDa. It acts as a serine/threonine phosphatase without intrinsic catalytic phosphoglycerate mutase activity. PGAM5 localizes to the inner membrane of mitochondria and exhibits expression in tissues where metabolic regulation is significant. It plays a role in removing phosphate groups from target substrates influencing multiple cellular mechanisms.

Biological function summary

PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.

Pathways

Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

PGAM5 relates to neurodegenerative diseases and cancer. Mutations or dysregulation of PGAM5 connect to Parkinson's disease where impaired mitochondrial function and mitophagy play a role. In cancer altered PGAM5 activity may disrupt normal apoptotic processes leading to unchecked cell growth. In these contexts PGAM5's interaction with proteins like PINK1 PARKIN RIPK1 and RIPK3 underpins its relevance to disease mechanisms.

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9 product images

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in HeLa wild-type cells (ab255928). Loss of signal was observed when knockout cell line Human PGAM5 knockout HeLa cell line ab265141 (knockout cell lysate Human PGAM5 knockout HeLa cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/mL
Lane 1: Wild-type HeLa lysate at 20 µg
Lane 2: PGAM5 knockout HeLa lysate at 20 µg
Lane 2: Western blot - Human PGAM5 knockout HeLa cell line (Human PGAM5 knockout HeLa cell line ab265141)
Lane 3: HepG2 lysate at 20 µg
Lane 4: Daudi lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human small intestine tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human tonsil tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human uterus tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human testis tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human liver tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
Paraffin-embedded human kidney tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
All lanes: Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate with siRNA control
Lane 2: RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 1
Lane 3: RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 2
Predicted band size: 32 kDa
Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
All lanes: Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Predicted band size: 32 kDa