AB244218

Anti-PGAM5 antibody [CL0624]

KO Validated
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(4 Publications)

Mouse Monoclonal PGAM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGAM5 aa 50-150.

View Alternative Names

Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, PGAM5

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human small intestine tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human uterus tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human kidney tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human liver tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human testis tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Paraffin-embedded human tonsil tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate with siRNA control

Lane 2:

RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 1

Lane 3:

RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 2

Predicted band size: 32 kDa

false

Lab

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in HeLa wild-type cells (ab255928). Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/mL

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

PGAM5 knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human PGAM5 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pgam5-knockout-hela-cell-line-ab265141'>ab265141</a>)

Lane 3:

HepG2 lysate at 20 µg

Lane 4:

Daudi lysate at 20 µg

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0624

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PGAM5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q96HS1

Epitope

Binds to an epitope located within the peptidesequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGAM5 also known as Phosphoglycerate Mutase Family Member 5 is a mitochondrial protein with a known mass of around 32 kDa. It acts as a serine/threonine phosphatase without intrinsic catalytic phosphoglycerate mutase activity. PGAM5 localizes to the inner membrane of mitochondria and exhibits expression in tissues where metabolic regulation is significant. It plays a role in removing phosphate groups from target substrates influencing multiple cellular mechanisms.

Biological function summary

PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.

Pathways

Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.

PGAM5 relates to neurodegenerative diseases and cancer. Mutations or dysregulation of PGAM5 connect to Parkinson's disease where impaired mitochondrial function and mitophagy play a role. In cancer altered PGAM5 activity may disrupt normal apoptotic processes leading to unchecked cell growth. In these contexts PGAM5's interaction with proteins like PINK1 PARKIN RIPK1 and RIPK3 underpins its relevance to disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed : 24746696, PubMed : 32439975). Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1 (PubMed : 32439975). Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation (PubMed : 37498743). Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3 (PubMed : 24746696). Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2 (PubMed : 18387606). Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria (PubMed : 22265414).

See full target information PGAM5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Medicine 102:e35452 PubMed37800782

2023

NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Tan,Yuqi He,Linhui Wu,Shiquan Liu,Peng Peng,Jiean Huang

Experimental and therapeutic medicine 23:359 PubMed35493422

2022

Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Xia,Wei Wang,Bing Gao,Qiang Ma,Jing Wang,Xiaohua Dai,Qingling Li

Redox biology 52:102301 PubMed35358851

2022

MnTE-2-PyP protects fibroblast mitochondria from hyperglycemia and radiation exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Arpita Chatterjee,Isin T Sakallioglu,Divya Murthy,Elizabeth A Kosmacek,Pankaj K Singh,J Tyson McDonald,Robert Powers,Rebecca E Oberley-Deegan

International journal of molecular sciences 22: PubMed34360749

2021

Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Csaba Horvath,Megan Young,Izabela Jarabicova,Lucia Kindernay,Kristina Ferenczyova,Tanya Ravingerova,Martin Lewis,M Saadeh Suleiman,Adriana Adameova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGAM5 antibody [CL0624]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (AB244218)

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

Western blot - Anti-PGAM5 antibody [CL0624] (AB244218)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise