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Knockout Tested Rabbit Recombinant Monoclonal PGAM5 antibody. Suitable for ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody [EPR27145-82] (AB308449), expandable thumbnail
  • Immunoprecipitation - Anti-PGAM5 antibody [EPR27145-82] (AB308449), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PGAM5 antibody [EPR27145-82] (AB308449), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)IPIHC-PIHC-Fr
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed:24746696, PubMed:32439975). Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1 (PubMed:32439975). Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation (PubMed:37498743). Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3 (PubMed:24746696). Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2 (PubMed:18387606). Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria (PubMed:22265414).

Alternative names

Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, PGAM5

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal PGAM5 antibody. Suitable for ICC/IF, Flow Cyt (Intra), IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27145-82
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PGAM5 also known as Phosphoglycerate Mutase Family Member 5 is a mitochondrial protein with a known mass of around 32 kDa. It acts as a serine/threonine phosphatase without intrinsic catalytic phosphoglycerate mutase activity. PGAM5 localizes to the inner membrane of mitochondria and exhibits expression in tissues where metabolic regulation is significant. It plays a role in removing phosphate groups from target substrates influencing multiple cellular mechanisms.

Biological function summary

PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.

Pathways

Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.

Associated diseases and disorders

PGAM5 relates to neurodegenerative diseases and cancer. Mutations or dysregulation of PGAM5 connect to Parkinson's disease where impaired mitochondrial function and mitophagy play a role. In cancer altered PGAM5 activity may disrupt normal apoptotic processes leading to unchecked cell growth. In these contexts PGAM5's interaction with proteins like PINK1 PARKIN RIPK1 and RIPK3 underpins its relevance to disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody [EPR27145-82] (ab308449), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody [EPR27145-82] (ab308449)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PGAM5 KO HeLa (PGAM5 knockout human cervix adenocarcinoma epithelial cell)(Human PGAM5 knockout HeLa cell line ab265141) cells labelling PGAM5 with ab308449 at 1/250 (2.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in parental HeLa, and no staining in PGAM5 KO HeLa cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-PGAM5 antibody [EPR27145-82] (ab308449), expandable thumbnail

    Immunoprecipitation - Anti-PGAM5 antibody [EPR27145-82] (ab308449)

    PGAM5 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab308449 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308449 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: abAB308449 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308449 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-PGAM5 antibody [EPR27145-82] (ab308449) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 32 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-PGAM5 antibody [EPR27145-82] (ab308449), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGAM5 antibody [EPR27145-82] (ab308449)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PGAM5 KO HeLa (PGAM5 knockout human cervix adenocarcinoma epithelial cell) (Human PGAM5 knockout HeLa cell line ab265141) (Left) / Parental HeLa (Right) cells labelling PGAM5 with ab308449 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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