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AB182569

Anti-PGAP3 antibody [EPR14380]

0

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal PGAP3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CAB2, PERLD1, UNQ546/PRO1100, PGAP3, GPI-specific phospholipase A2-like PGAP3, COS16 homolog, Gene coamplified with ERBB2 protein, PER1-like domain-containing protein 1, Post-GPI attachment to proteins factor 3, hCOS16

2 Images
Western blot - Anti-PGAP3 antibody [EPR14380] (AB182569)
  • WB

Supplier Data

Western blot - Anti-PGAP3 antibody [EPR14380] (AB182569)

All lanes:

Western blot - Anti-PGAP3 antibody [EPR14380] (ab182569) at 1/5000 dilution

Lane 1:

Human thyroid tissue lysate at 10 µg

Lane 2:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 36 kDa

false

Western blot - Anti-PGAP3 antibody [EPR14380] (AB182569)
  • WB

Supplier Data

Western blot - Anti-PGAP3 antibody [EPR14380] (AB182569)

All lanes:

Western blot - Anti-PGAP3 antibody [EPR14380] (ab182569) at 1/5000 dilution

Lane 1:

THP1 cell line lysate at 20 µg

Lane 2:

HeLa cell line lysate at 20 µg

Lane 3:

HepG2 cell line lysate at 20 µg

Lane 4:

JAR cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 36 kDa

false

  • 805 Alexa Fluor® 790

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14380

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target PGAP3 also called post-GPI attachment to proteins 3 is a protein involved in the processing of glycosylphosphatidylinositol (GPI) anchors. It is encoded by the PGAP3 gene and has a molecular mass of around 104 kDa. This protein expresses highly in various tissues especially in the brain and testis. PGAP3 plays an important role in the correct remodeling of GPI anchors which are critical for the stable attachment of proteins to the cell membrane.
Biological function summary

PGAP3 removes unsaturated fatty acids from GPI anchors' lipid tails allowing the addition of saturated fatty acids. This process is essential for the function and stability of GPI-anchored proteins. Although not a member of a large protein complex PGAP3 works closely with other enzymes involved in GPI-anchor maturation. The remodeling of GPI anchors by PGAP3 ensures that proteins can adhere properly to the cell membrane and perform their functions.

Pathways

The correct localization of GPI-anchored proteins depends heavily on PGAP3 function. The protein plays an important role in the GPI-anchor biosynthesis pathway and changes in PGAP3 activity affect this pathway significantly. The remodeling of GPI anchors connects PGAP3 to proteins like PIG-N which also participates in this biosynthesis process. By ensuring the correct GPI anchor assembly PGAP3 helps maintain cellular communication and signaling pathways.

Alterations in PGAP3 function associate with conditions such as intellectual disability and GPI-anchor deficiency syndrome. Mutations in the PGAP3 gene disrupt normal GPI-anchor maturation leading to a range of developmental and neurological abnormalities. The protein is also linked with PIGT another enzyme involved in the GPI biosynthesis pathway implicating these proteins in the onset of specific disorders when disrupted. Understanding the role of PGAP3 in these diseases aids in developing potential targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the fatty acid remodeling steps of GPI-anchor maturation where the unsaturated acyl chain at sn-2 of inositol phosphate is replaced by a saturated stearoyl chain (PubMed : 17021251, PubMed : 24439110). May catalyze the first step of the fatty acid remodeling, by removing the unsaturated acyl chain at sn-2 of inositol phosphate, generating a lyso-GPI intermediate (Probable). The fatty acid remodeling steps is critical for the integration of GPI-APs into lipid rafts (By similarity).
See full target information PGAP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 19:237 PubMed34078403

2021

Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Ónody,Apor Veres-Székely,Domonkos Pap,Réka Rokonay,Beáta Szebeni,Erna Sziksz,Franz Oswald,Gábor Veres,Áron Cseh,Attila J Szabó,Ádám Vannay
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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