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AB250622

Anti-PGAP3 antibody [EPR14380] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PGAP3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CAB2, PERLD1, UNQ546/PRO1100, PGAP3, Post-GPI attachment to proteins factor 3, COS16 homolog, Gene coamplified with ERBB2 protein, PER1-like domain-containing protein 1, hCOS16

2 Images
Western blot - Anti-PGAP3 antibody [EPR14380] - BSA and Azide free (AB250622)
  • WB

Supplier Data

Western blot - Anti-PGAP3 antibody [EPR14380] - BSA and Azide free (AB250622)

This data was developed using ab182569, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGAP3 antibody [EPR14380] (<a href='/en-us/products/primary-antibodies/pgap3-antibody-epr14380-ab182569'>ab182569</a>) at 1/5000 dilution

Lane 1:

Human thyroid tissue lysate at 10 µg

Lane 2:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 36 kDa

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Western blot - Anti-PGAP3 antibody [EPR14380] - BSA and Azide free (AB250622)
  • WB

Supplier Data

Western blot - Anti-PGAP3 antibody [EPR14380] - BSA and Azide free (AB250622)

This data was developed using ab182569, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGAP3 antibody [EPR14380] (<a href='/en-us/products/primary-antibodies/pgap3-antibody-epr14380-ab182569'>ab182569</a>) at 1/5000 dilution

Lane 1:

THP1 cell line lysate at 20 µg

Lane 2:

HeLa cell line lysate at 20 µg

Lane 3:

HepG2 cell line lysate at 20 µg

Lane 4:

JAR cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14380

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250622 is the carrier-free version of ab182569.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target PGAP3 also called post-GPI attachment to proteins 3 is a protein involved in the processing of glycosylphosphatidylinositol (GPI) anchors. It is encoded by the PGAP3 gene and has a molecular mass of around 104 kDa. This protein expresses highly in various tissues especially in the brain and testis. PGAP3 plays an important role in the correct remodeling of GPI anchors which are critical for the stable attachment of proteins to the cell membrane.
Biological function summary

PGAP3 removes unsaturated fatty acids from GPI anchors' lipid tails allowing the addition of saturated fatty acids. This process is essential for the function and stability of GPI-anchored proteins. Although not a member of a large protein complex PGAP3 works closely with other enzymes involved in GPI-anchor maturation. The remodeling of GPI anchors by PGAP3 ensures that proteins can adhere properly to the cell membrane and perform their functions.

Pathways

The correct localization of GPI-anchored proteins depends heavily on PGAP3 function. The protein plays an important role in the GPI-anchor biosynthesis pathway and changes in PGAP3 activity affect this pathway significantly. The remodeling of GPI anchors connects PGAP3 to proteins like PIG-N which also participates in this biosynthesis process. By ensuring the correct GPI anchor assembly PGAP3 helps maintain cellular communication and signaling pathways.

Alterations in PGAP3 function associate with conditions such as intellectual disability and GPI-anchor deficiency syndrome. Mutations in the PGAP3 gene disrupt normal GPI-anchor maturation leading to a range of developmental and neurological abnormalities. The protein is also linked with PIGT another enzyme involved in the GPI biosynthesis pathway implicating these proteins in the onset of specific disorders when disrupted. Understanding the role of PGAP3 in these diseases aids in developing potential targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI.
See full target information PGAP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu
View all publications
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Product promise

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