AB180598

Anti-PGBD1 antibody [EPR13883]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PGBD1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

hucep-4, PGBD1, PiggyBac transposable element-derived protein 1, Cerebral protein 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] (AB180598)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] (AB180598)

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling PGBD1 with ab180598 at a 1/500 dilution (left image) and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] (AB180598)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] (AB180598)

Intracellular Flow Cytometry analysis of HeLa cells using ab180598at a 1/20 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary usedat a 1/150 dilution.

Supplier Data

Western blot - Anti-PGBD1 antibody [EPR13883] (AB180598)

All lanes:

Western blot - Anti-PGBD1 antibody [EPR13883] (ab180598) at 1/10000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Lane 3:

293 lysate at 20 µg

Lane 4:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution

Predicted band size: 93 kDa

false

Carrier free

Anti-PGBD1 antibody [EPR13883] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13883

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGBD1 also known as PiggyBac Transposable Element-Derived 1 is a DNA transposase-like protein. The human PGBD1 protein has a molecular mass of approximately 98 kDa. It is a member of the piggyBac family of transposases and is highly expressed in the brain and various other tissues. PGBD1 has a sequence that shows homology to known active transposases but lacks the catalytic triad of DDD signature suggesting a more complex role beyond simple transposition mechanics.

Biological function summary

The gene encoding PGBD1 is involved in processes related to genomic stability and chromatin remodeling although it does not form part of a known multiprotein complex. This protein plays roles in gene expression regulation and might affect chromatin through non-transposase activity. It is hypothesized that PGBD1 might bind to specific DNA sequences impacting chromatin state and gene activity. Its exact biological function remains under investigation but the regulation of neural-related genes implicates a role in neural tissue development and maintenance.

Pathways

PGBD1 is involved in the regulation of chromatin structure and gene expression pathways. Its interactions influence pathways like the DNA damage response contributing to cellular reactions to stress at the genetic level. The protein may not operate alone but has functional associations with chromatin remodelers such as Polycomb group proteins which are important in managing gene expression patterns during development and differentiation.

Dysregulation or mutations of PGBD1 suggest potential links with neurodevelopmental disorders and cancer. Altered expression patterns have been noted in neuroblastoma implying interactions with proteins like MYCN an important oncogene. PGBD1's effect on chromatin dynamics could influence the genomic environment leading to disease-associated changes in gene expression. Further research seeks to clarify these connections and determine potential therapeutic targets or biomarkers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transposase-derived from PiggyBac DNA transposons. Although it has been fully domesticated and lacks transposase activity, PGBD1 has acquired DNA-binding capability (PubMed : 12955498, PubMed : 35609796, PubMed : 36205081). It preferentially binds in and around genes involved in neuronal development, leading to their transcriptional pausing. Notably, PGBD1 suppresses paraspeckle assembly in neuronal cells (PubMed : 36205081).

See full target information PGBD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 645:1011-1019 PubMed40804518

2025

Multiple oestradiol functions inhibit ferroptosis and acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wulf Tonnus,Francesca Maremonti,Shubhangi Gavali,Marlena Nastassja Schlecht,Florian Gembardt,Alexia Belavgeni,Nadja Leinung,Karolin Flade,Natalie Bethe,Sofia Traikov,Anne Haag,Danny Schilling,Sider Penkov,Melodie Mallais,Christine Gaillet,Claudia Meyer,Melika Katebi,Anushka Ray,Louisa M S Gerhardt,Anne Brucker,Jorunn Naila Becker,Mirela Tmava,Lisa Schlicker,Almut Schulze,Nina Himmerkus,Andrej Shevchenko,Mirko Peitzsch,Uladzimir Barayeu,Sonia Nasi,Juliane Putz,Kenneth S Korach,Joel Neugarten,Ladan Golestaneh,Christian Hugo,Jan Ulrich Becker,Joel M Weinberg,Svenja Lorenz,Bettina Proneth,Marcus Conrad,Eckhard Wolf,Bernd Plietker,Raphaël Rodriguez,Derek A Pratt,Tobias P Dick,Maria Fedorova,Stefan R Bornstein,Andreas Linkermann

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300383 PubMed37340596

2023

A New Type of Endometrial Cancer Models in Mice Revealing the Functional Roles of Genetic Drivers and Exploring their Susceptibilities.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyao Chen,Siqi Dai,Lei Zhao,Yiman Peng,Chongen Sun,Hongling Peng,Qian Zhong,Yuan Quan,Yue Li,Xuelan Chen,Xiangyu Pan,Ailing Zhong,Manli Wang,Mengsha Zhang,Shengyong Yang,You Lu,Zhong Lian,Yu Liu,Shengtao Zhou,Zhengyu Li,Feifei Na,Chong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGBD1 antibody [EPR13883]

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] (AB180598)

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] (AB180598)

Western blot - Anti-PGBD1 antibody [EPR13883] (AB180598)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise