AB250242

Anti-PGBD1 antibody [EPR13883] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PGBD1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

hucep-4, PGBD1, PiggyBac transposable element-derived protein 1, Cerebral protein 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

This data was developed using ab180598, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling PGBD1 with ab180598 at a 1/500 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

This data was developed using ab180598, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells using ab180598 at a 1/20 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

Supplier Data

Western blot - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

This data was developed using ab180598, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGBD1 antibody [EPR13883] (<a href='/en-us/products/primary-antibodies/pgbd1-antibody-epr13883-ab180598'>ab180598</a>) at 1/10000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

Jurkat lysate at 20 µg

Lane 3:

293 lysate at 20 µg

Lane 4:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution

Predicted band size: 93 kDa

false

Unconjugated

Anti-PGBD1 antibody [EPR13883]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13883

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250242 is the carrier-free version of ab180598.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGBD1 also known as PiggyBac Transposable Element-Derived 1 is a DNA transposase-like protein. The human PGBD1 protein has a molecular mass of approximately 98 kDa. It is a member of the piggyBac family of transposases and is highly expressed in the brain and various other tissues. PGBD1 has a sequence that shows homology to known active transposases but lacks the catalytic triad of DDD signature suggesting a more complex role beyond simple transposition mechanics.

Biological function summary

The gene encoding PGBD1 is involved in processes related to genomic stability and chromatin remodeling although it does not form part of a known multiprotein complex. This protein plays roles in gene expression regulation and might affect chromatin through non-transposase activity. It is hypothesized that PGBD1 might bind to specific DNA sequences impacting chromatin state and gene activity. Its exact biological function remains under investigation but the regulation of neural-related genes implicates a role in neural tissue development and maintenance.

Pathways

PGBD1 is involved in the regulation of chromatin structure and gene expression pathways. Its interactions influence pathways like the DNA damage response contributing to cellular reactions to stress at the genetic level. The protein may not operate alone but has functional associations with chromatin remodelers such as Polycomb group proteins which are important in managing gene expression patterns during development and differentiation.

Dysregulation or mutations of PGBD1 suggest potential links with neurodevelopmental disorders and cancer. Altered expression patterns have been noted in neuroblastoma implying interactions with proteins like MYCN an important oncogene. PGBD1's effect on chromatin dynamics could influence the genomic environment leading to disease-associated changes in gene expression. Further research seeks to clarify these connections and determine potential therapeutic targets or biomarkers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PGBD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGBD1 antibody [EPR13883] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

Flow Cytometry (Intracellular) - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

Western blot - Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (AB250242)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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