AB77210

Anti-PGC1 alpha antibody [4A8]

Name: Anti-PGC1 alpha antibody [4A8] (ab77210) | Abcam
Brand: Abcam Limited
SKU: AB77210
Price: 715 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(20 Publications)

Mouse Monoclonal PGC1 alpha antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPARGC1A aa 650 to C-terminus.

View Alternative Names

peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta

3 Images

Flow Cytometry - Anti-PGC1 alpha antibody [4A8] (AB77210)

Flow Cyt

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Flow Cytometry - Anti-PGC1 alpha antibody [4A8] (AB77210)

Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

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Western blot - Anti-PGC1 alpha antibody [4A8] (AB77210)

Western blot against tagged recombinant protein immunogen using ab77210 antibody at 1/500 dilution. Predicted band size of immunogen is 92kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/mL

All lanes:

Recombinant fragment of human PGC1 alpha at 0.1 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 91 kDa

Observed band size: 38 kDa

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Western blot - Anti-PGC1 alpha antibody [4A8] (AB77210)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/mL

All lanes:

PGC1 alpha transfected U2OS cell lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 91 kDa

Observed band size: 45 kDa,91 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A8

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human PPARGC1A aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBK2

Specificity

The immunogen sequence for this antibody is 55% homologous with PGC1 beta (Uniprot: Q86YN6).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Constituents: PBS, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PGC1 alpha also known as peroxisome proliferator-activated receptor gamma coactivator 1-alpha has a molecular weight of around 91 kDa. It functions mechanically as a transcription coactivator activating transcriptional protein pathways by binding to various transcription factors. It expresses in tissues with high energy demands notably in brown adipose tissue muscle liver heart and kidney. Scientists often investigate PGC1 alpha through various methods including PGC1 alpha Western blot analysis.

Biological function summary

PGC1 alpha plays a significant role in regulating genes involved in energy metabolism. This protein exists as part of larger transcription complexes interacting with nuclear receptors to control mitochondrial biogenesis and activity. It enhances oxidative phosphorylation and adaptive thermogenesis adapting to changes in energy balance. PGC1 alpha is also important in the regulation of blood pressure cholesterol homeostasis and fatty acid oxidation.

Pathways

PGC1 alpha interacts with several key metabolic pathways. It is involved in the regulation of the PPAR (peroxisome proliferator-activated receptor) pathway impacting both lipid metabolism and glucose homeostasis. Furthermore PGC1 alpha influences the activity of transcription factors like NRF1 and NRF2 which play important roles in cellular energy homeostasis and response to oxidative stress.

PGC1 alpha is linked to metabolic conditions such as type 2 diabetes and obesity. It interacts with proteins like PPAR gamma relevant in these metabolic disorders. Changes in PGC1 alpha expression or function can disrupt energy balance and mitochondrial function contributing to the pathophysiology of these diseases by impairing metabolic regulation and increasing susceptibility to insulin resistance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Acts as a key regulator of gluconeogenesis : stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed : 16753578, PubMed : 23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed : 23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).

See full target information PPARGC1A

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:21 PubMed39856069

2025

Autophagic flux-lipid droplet biogenesis cascade sustains mitochondrial fitness in colorectal cancer cells adapted to acidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Liu,Xue Sun,Wenqing Mu,Yanan Li,Wenqing Bu,Tingting Yang,Jia Zhang,Rui Liu,Jiayu Ren,Jin Zhou,Peishan Li,Yufang Shi,Changshun Shao

Redox report : communications in free radical research 29:2433393 PubMed39620924

2024

Sodium formate induces development-dependent intestinal epithelial injury via necroptosis and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wei,Yuan Tian,Meiqi Guan,Jinshu Wei,Yong Ji,Guozhong Tao,Karl G Sylvester

Cells 13: PubMed38667324

2024

The Role of -Adrenergic Receptors in Cold-Induced Beige Adipocyte Production in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Yang,Hong Ma,Liang Wang,Fang Wang,Jiqiao Xia,Dongyu Liu,Linlin Mu,Xiuqin Yang,Di Liu

International journal of molecular sciences 24: PubMed37833939

2023

(+)-Lipoic Acid Reduces Lipotoxicity and Regulates Mitochondrial Homeostasis and Energy Balance in an In Vitro Model of Liver Steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Longhitano,Alfio Distefano,Angela Maria Amorini,Laura Orlando,Sebastiano Giallongo,Daniele Tibullo,Giuseppe Lazzarino,Anna Nicolosi,Amer M Alanazi,Concetta Saoca,Vincenzo Macaione,M'hammed Aguennouz,Federico Salomone,Emanuela Tropea,Ignazio Alberto Barbagallo,Giovanni Li Volti,Giacomo Lazzarino

Cell cycle (Georgetown, Tex.) 21:2206-2221 PubMed35815665

2022

Comparing the mitochondrial signatures in ESCs and iPSCs and their neural derivations.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilie Katrin Kristiansen,Anbin Chen,Lena Elise Høyland,Mathias Ziegler,Gareth John Sullivan,Laurence A Bindoff,Kristina Xiao Liang

Blood advances 6:4185-4195 PubMed35580333

2022

Depletion of CLL cells by venetoclax treatment reverses oxidative stress and impaired glycolysis in CD4 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

J A C van Bruggen,G J W van der Windt,M Hoogendoorn,J Dubois,Arnon P Kater,F S Peters

Journal of inflammation (London, England) 18:31 PubMed34930287

2021

Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Wu,Xiao Yu,Yue Peng,Qian-Lu Wang,Long-Tian Deng,Wei Xing

Cancers 13: PubMed34680366

2021

Identification of Biochemical and Molecular Markers of Early Aging in Childhood Cancer Survivors.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Ravera,Tiziana Vigliarolo,Silvia Bruno,Fabio Morandi,Danilo Marimpietri,Federica Sabatini,Monica Dagnino,Andrea Petretto,Martina Bartolucci,Monica Muraca,Eleonora Biasin,Riccardo Haupt,Marco Zecca,Franca Fagioli,Daniela Cilloni,Marina Podestà,Francesco Frassoni

Immunity, inflammation and disease 9:710-720 PubMed34078001

2021

Ferulic acid suppresses interleukin-1β-induced degeneration of chondrocytes isolated from patients with osteoarthritis through the SIRT1/AMPK/PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kewei Du,Xuchen Fang,Ziqiang Li

Neurobiology of disease 148:105162 PubMed33171227

2020

PPAR gamma agonist leriglitazone improves frataxin-loss impairments in cellular and animal models of Friedreich Ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Rodríguez-Pascau,Elena Britti,Pablo Calap-Quintana,Yi Na Dong,Cristina Vergara,Fabien Delaspre,Marta Medina-Carbonero,Jordi Tamarit,Federico V Pallardó,Pilar Gonzalez-Cabo,Joaquim Ros,David R Lynch,Marc Martinell,Pilar Pizcueta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGC1 alpha antibody [4A8]

Flow Cytometry - Anti-PGC1 alpha antibody [4A8] (AB77210)

Western blot - Anti-PGC1 alpha antibody [4A8] (AB77210)

Western blot - Anti-PGC1 alpha antibody [4A8] (AB77210)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

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