Goat Polyclonal PGC1 alpha antibody. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PPARGC1A.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPARGC1A. Database link Q9UBK2
Reactivity data
Select an application| WB | ICC/IF | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.5-3 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-6 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Target data
Function
Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed:10713165, PubMed:20005308, PubMed:21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed:10713165, PubMed:20005308, PubMed:21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed:10713165, PubMed:20005308, PubMed:21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed:10713165, PubMed:20005308, PubMed:21376232). Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed:16753578, PubMed:23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed:23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).
Alternative names
peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta
Recommended products
Goat Polyclonal PGC1 alpha antibody. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PPARGC1A.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPARGC1A. Database link Q9UBK2
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody appears to recognise multiple isoforms.
NP_037393.1, NP_001317680.1, NP_001317681.1, NP_001317682.1 , NP_001341756.1.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PGC1 alpha also known as peroxisome proliferator-activated receptor gamma coactivator 1-alpha has a molecular weight of around 91 kDa. It functions mechanically as a transcription coactivator activating transcriptional protein pathways by binding to various transcription factors. It expresses in tissues with high energy demands notably in brown adipose tissue muscle liver heart and kidney. Scientists often investigate PGC1 alpha through various methods including PGC1 alpha Western blot analysis.
- Biological function summary
PGC1 alpha plays a significant role in regulating genes involved in energy metabolism. This protein exists as part of larger transcription complexes interacting with nuclear receptors to control mitochondrial biogenesis and activity. It enhances oxidative phosphorylation and adaptive thermogenesis adapting to changes in energy balance. PGC1 alpha is also important in the regulation of blood pressure cholesterol homeostasis and fatty acid oxidation.
- Pathways
PGC1 alpha interacts with several key metabolic pathways. It is involved in the regulation of the PPAR (peroxisome proliferator-activated receptor) pathway impacting both lipid metabolism and glucose homeostasis. Furthermore PGC1 alpha influences the activity of transcription factors like NRF1 and NRF2 which play important roles in cellular energy homeostasis and response to oxidative stress.
- Associated diseases and disorders
PGC1 alpha is linked to metabolic conditions such as type 2 diabetes and obesity. It interacts with proteins like PPAR gamma relevant in these metabolic disorders. Changes in PGC1 alpha expression or function can disrupt energy balance and mitochondrial function contributing to the pathophysiology of these diseases by impairing metabolic regulation and increasing susceptibility to insulin resistance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody (ab317540)
ab317540 (5 µg/ml) staining of paraffin embedded human kidney.
Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Inset - Negative control showing staining of paraffin embedded human kidney, with no primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody (ab317540)
ab317540 (6 µg/ml) staining of paraffin embedded human liver.
Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Inset - Negative control showing staining of paraffin embedded human liver, with no primary antibody.
Flow Cytometry (Intracellular) - Anti-PGC1 alpha antibody (ab317540)
Flow cytometric (Intracellular) analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (1 ug/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (ab317540)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml).
Western blot - Anti-PGC1 alpha antibody (ab317540)
Primary incubation 1 hour at room temperature.
Detected by chemiluminescence.
Approx. 70+90kDa bands observed in lysates of cell line A431 and in nuclear HEK293 cell lysates, and an additional 85kDa band observed in HepG2 cell lysate (calculated MW of 91.0kDa according to NP_037393.1, 89.6kDa according to NP_001317681.1, and 77.1kDa according to NP_001317682.1).
All bands were successfully blocked by incubation with the immunizing peptide.Lanes 1 - 2: Western blot - Anti-PGC1 alpha antibody (ab317540) at 3 µg/mL
Lane 3: Western blot - Anti-PGC1 alpha antibody (ab317540) at 1.5 µg/mL
Lane 1: A431 nuclear lysate in RIPA buffer at 35 µg
Lane 2: HepG2 nuclear lysate in RIPA buffer at 35 µg
Lane 3: HEK293 nuclear lysate in RIPA buffer at 35 µg
Predicted band size: 91 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (ab317540)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml).
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