Anti-PGC1 alpha antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody (AB317540)

ab317540 (6 µg/ml) staining of paraffin embedded human liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Inset - Negative control showing staining of paraffin embedded human liver, with no primary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (AB317540)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (AB317540)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (2 ug/ml).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PGC1 alpha antibody (AB317540)

Flow cytometric (Intracellular) analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® secondary antibody (1 ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488® secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody (AB317540)

ab317540 (5 µg/ml) staining of paraffin embedded human kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Inset - Negative control showing staining of paraffin embedded human kidney, with no primary antibody.

• WB

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Western blot - Anti-PGC1 alpha antibody (AB317540)

Primary incubation 1 hour at room temperature. Detected by chemiluminescence. Approx. 70+90kDa bands observed in lysates of cell line A431 and in nuclear HEK293 cell lysates, and an additional 85kDa band observed in HepG2 cell lysate (calculated MW of 91.0kDa according to NP_037393.1, 89.6kDa according to NP_001317681.1, and 77.1kDa according to NP_001317682.1). All bands were successfully blocked by incubation with the immunizing peptide.

Lanes 1 - 2:

Western blot - Anti-PGC1 alpha antibody (ab317540) at 3 µg/mL

Lane 3:

Western blot - Anti-PGC1 alpha antibody (ab317540) at 1.5 µg/mL

Lane 1:

A431 nuclear lysate in RIPA buffer at 35 µg

Lane 2:

HepG2 nuclear lysate in RIPA buffer at 35 µg

Lane 3:

HEK293 nuclear lysate in RIPA buffer at 35 µg

Predicted band size: 91 kDa

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