Skip to main content

Rabbit Recombinant Monoclonal PGC1 alpha antibody. Suitable for WB, Dot and reacts with Mouse, Rat, Synthetic peptide samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (AB313559), expandable thumbnail
  • Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (AB313559), expandable thumbnail
  • Dot Blot - Anti-PGC1 alpha antibody [EPR25162-281] (AB313559), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIHC-PIHC-FrIPICC/IFFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed:10713165, PubMed:20005308, PubMed:21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed:10713165, PubMed:20005308, PubMed:21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed:10713165, PubMed:20005308, PubMed:21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed:10713165, PubMed:20005308, PubMed:21376232). Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed:16753578, PubMed:23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed:23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).

Alternative names

peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta

Recommended products

Rabbit Recombinant Monoclonal PGC1 alpha antibody. Suitable for WB, Dot and reacts with Mouse, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25162-281
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our broad range of gold-standard reagents features highly specific recombinant antibodies and kits, validated across applications and species to ensure the precision and reproducibility your work requires. Drive your drug discovery efforts forward with our tailored solutions designed to meet the unique challenges of obesity research.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein PGC1 alpha also known as peroxisome proliferator-activated receptor gamma coactivator 1-alpha has a molecular weight of around 91 kDa. It functions mechanically as a transcription coactivator activating transcriptional protein pathways by binding to various transcription factors. It expresses in tissues with high energy demands notably in brown adipose tissue muscle liver heart and kidney. Scientists often investigate PGC1 alpha through various methods including PGC1 alpha Western blot analysis.

Biological function summary

PGC1 alpha plays a significant role in regulating genes involved in energy metabolism. This protein exists as part of larger transcription complexes interacting with nuclear receptors to control mitochondrial biogenesis and activity. It enhances oxidative phosphorylation and adaptive thermogenesis adapting to changes in energy balance. PGC1 alpha is also important in the regulation of blood pressure cholesterol homeostasis and fatty acid oxidation.

Pathways

PGC1 alpha interacts with several key metabolic pathways. It is involved in the regulation of the PPAR (peroxisome proliferator-activated receptor) pathway impacting both lipid metabolism and glucose homeostasis. Furthermore PGC1 alpha influences the activity of transcription factors like NRF1 and NRF2 which play important roles in cellular energy homeostasis and response to oxidative stress.

Associated diseases and disorders

PGC1 alpha is linked to metabolic conditions such as type 2 diabetes and obesity. It interacts with proteins like PPAR gamma relevant in these metabolic disorders. Changes in PGC1 alpha expression or function can disrupt energy balance and mitochondrial function contributing to the pathophysiology of these diseases by impairing metabolic regulation and increasing susceptibility to insulin resistance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559), expandable thumbnail

    Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: white fat (PMID: 9529258, 18728005).
    Low expression: brain(PMID: 9529258, 18728005).
    The molecular weight observed is consistent with what has been described in the literature (PMID: 29777200).Extra bands around 75 kDa and 36KDa were observed.
    In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559) at 1/1000 dilution

    Lane 1: Mouse brown fat tissue lysate at 20 µg

    Lane 2: Mouse white fat tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 113 kDa

    Exposure time: 26s

  • Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559), expandable thumbnail

    Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: white fat, lung (PMID: 9529258, 18728005).
    The molecular weight observed is consistent with what has been described in the literature (PMID: 29777200, 28611589).Extra bands around 75 kDa and 36KDa were observed.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: Lane 1-4: 15 seconds; Lane 5-6: 48 seconds

    All lanes: Western blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 20 µg

    Lane 2: Rat heart tissue lysate at 20 µg

    Lane 3: Rat brown fat tissue lysate at 20 µg

    Lane 4: Rat white fat tissue lysate at 20 µg

    Lane 5: Rat kidney tissue lysate at 20 µg

    Lane 6: Rat lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 113 kDa

  • Dot Blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559), expandable thumbnail

    Dot Blot - Anti-PGC1 alpha antibody [EPR25162-281] (ab313559)

    Dot blot analysis of PGC1 alpha using ab313559 at 1:1000 (0.51 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Lane 1: PGC1 alpha peptide
    Lane 2: PGC1 beta peptide
    Exposure time: 180 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com