Rabbit Polyclonal PGC1 alpha antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide - Human, Mouse, Rat, Human samples. Cited in 43 publications.
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- Science advances 9:eadi27672023ANT-dependent MPTP underlies necrotic myofiber death in muscular dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J Bround,Julian R Havens,Allen J York,Michelle A Sargent,Jason Karch,Jeffery D MolkentinPubMed 37624892
- Nature communications 14:42572023The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Jin,Shuang Han,Xue Lv,Xiaofei Li,Ziyin Zhang,Henry Kuang,Zhimin Chen,Cheng-An Lv,Wei Peng,Zhuoying Yang,Miqi Yang,Lin Mi,Tongyu Liu,Shengshan Ma,Xinyuan Qiu,Qintao Wang,Xiaowen Pan,Pengfei Shan,Yu Feng,Jin Li,Fudi Wang,Liwei Xie,Xuyun Zhao,Jun-Fen Fu,Jiandie D Lin,Zhuo-Xian MengPubMed 37468484
- Disease models & mechanisms 16:2023A gut microbiome metabolite paradoxically depresses contractile function while activating mitochondrial respiration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaba Naghipour,Joshua J Fisher,Anthony V Perkins,Jason N Peart,John P Headrick,Eugene F Du ToitPubMed 37078591
- Biological & pharmaceutical bulletin 46:563-5732023PGC 1α-Mediates Mitochondrial Damage in the Liver by Inhibiting the Mitochondrial Respiratory Chain as a Non-cholinergic Mechanism of Repeated Low-Level Soman Exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Jin,Yi Zhang,Yalan Cui,Meng Shi,Jingjing Shi,Siqing Zhu,Tong Shi,Ruihua Zhang,Xuejun Chen,Xingxing Zong,Chen Wang,Liqin LiPubMed 37005300
- Phytotherapy research : PTR 36:2495-25102022Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Cheng,Lu Shi,Changhao He,Chen Wang,Yinglan Lv,Huimin Li,Yongcheng An,Hongyu Dai,Yuhui Duan,Huilin Zhang,Yan Huang,Wanxin Fu,Yanyan Meng,Baosheng ZhaoPubMed 35445769
- iScience 25:1041842022Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeroy C Joseph,Jianting Shi,Quynh N Nguyen,Victoria Pensiero,Chris Goulbourne,Robert C Bauer,Hanrui Zhang,John P MorrowPubMed 35494220
- Frontiers in pharmacology 12:6423982021Cannabinoid Receptor Agonist Inhibits Atrial Electrical Remodeling in a Tachypaced Rat Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle I Lee,Michael Murninkas,Sigal Elyagon,Yoram Etzion,Hope D AndersonPubMed 33967775
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e212232021The lysosomal membrane protein Sidt2 is a vital regulator of mitochondrial quality control in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLizhuo Wang,Cui Yu,Wenjun Pei,Mengya Geng,Yao Zhang,Zihui Li,Feiteng Liang,Fengbiao Tan,Hui Du,Jialin GaoPubMed 33715196
- European journal of pharmacology :1739182021Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNandini Ravikumar Jayakumari et. al.PubMed 33529726
- Cell reports 34:1085722021ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoyuan Qi,Yashi Mi,Xiaojian Shi,Haiwei Gu,Roberta Diaz Brinton,Fei YinPubMed 33406436
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | ICC/IF | |
|---|---|---|---|
| Human | Predicted | Expected | Expected |
| Mouse | Predicted | Tested | Expected |
| Rat | Predicted | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Synthetic peptide - Human | Expected | Not recommended | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 0.25000-1000.00000 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog, Human, Pig, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed:10713165, PubMed:20005308, PubMed:21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed:10713165, PubMed:20005308, PubMed:21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed:10713165, PubMed:20005308, PubMed:21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed:10713165, PubMed:20005308, PubMed:21376232). Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed:16753578, PubMed:23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed:23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).
Additional Targets
PPARGC1B
Alternative names
peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta
Recommended products
Rabbit Polyclonal PGC1 alpha antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide - Human, Mouse, Rat, Human samples. Cited in 43 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunogen design for ab72230 is shown to cross-react with both PGC1 alpha (ID: 124782 and Swissprot Q9UBK2) and PGC1 beta (ID: 240776 and SwissProt Q86YN6). From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab188102.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
PGC1 alpha has a predicted MW of 92 kDa and PGC1 beta has a predicted MW of 113 kDa.
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGC1 alpha and beta also known as Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC-1α) and 1-beta (PGC-1β) are transcriptional coactivators with molecular weights of approximately 91 kDa and 102 kDa respectively. These proteins are important in regulating energy metabolism and are highly expressed in tissues with high energy demand such as skeletal muscle heart and brown adipose tissue. The proteins interact with nuclear hormone receptors and various transcription factors to control the expression of genes involved in energy homeostasis.
- Biological function summary
PGC1 alpha and beta activate and coordinate the expression of genes involved in mitochondrial biogenesis and oxidative phosphorylation. They do not form a complex themselves but work with other transcriptional regulators to enhance gene expression. These proteins influence the metabolic processes of cells regulating the conversion of energy substrates into usable cellular energy. They are critical in adapting to different physiological conditions like exercise and fasting by modulating the capacity for energy production.
- Pathways
PGC1 alpha and beta integrate signals from various pathways like the PPAR and AMPK pathways which are significant for energy balance and metabolism. PGC1 alpha particularly interacts with proteins such as NRF1 and NRF2 to promote mitochondrial function. Through these pathways PGC1 coactivators help regulate metabolism by altering the transcriptional activity of genes involved in energy expenditure and metabolic adaptation.
- Associated diseases and disorders
PGC1 alpha and beta have significant implications in conditions such as obesity and diabetes. Dysregulation of these proteins affects the balance of energy metabolism leading to metabolic disorders. PGC1 alpha in particular interacts with proteins like SIRT1 which influence insulin sensitivity and metabolic rate thereby playing a role in the development and progression of these diseases. The modulation of PGC1 activity has potential therapeutic implications for managing metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (ab72230)
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.
Secondary ab: Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution. -ve control 2 is Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.
Western blot - Anti-PGC1 alpha + beta antibody (ab72230)
We have occasionally observed bands in the region of 90 kDa in batches of this antibody, which corresponds to the molecular weight based on primary sequence.
All lanes: Western blot - Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mL
Lane 1: Testis (Mouse) Tissue Lysate at 10 µg
Lane 2: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Lane 3: Ovary (Rat) Tissue Lysate at 10 µg
Lane 4: Colon (Mouse) Tissue Lysate at 10 µg
Lane 5: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg
Lane 6: Thymus (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 110 kDa, 35 kDa, 45 kDa, 55 kDa
Exposure time: 2min
Western blot - Anti-PGC1 alpha + beta antibody (ab72230)
Western Blot showing equal blocking with the PGC1 alpha and PGC1 beta peptides.
All lanes: Western blot - Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mL
Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Lane 2: Colon (Mouse) Tissue Lysate at 10 µg
Lane 3: Thymus (Rat) Tissue Lysate at 10 µg
Lane 4: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg with Human PGC1 alpha peptide (ab73600)
Lane 5: Colon (Mouse) Tissue Lysate at 10 µg with Human PGC1 alpha peptide (ab73600)
Lane 6: Thymus (Rat) Tissue Lysate at 10 µg with Human PGC1 alpha peptide (ab73600)
Lane 7: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg with PGC1 beta peptide
Lane 8: Colon (Mouse) Tissue Lysate at 10 µg with PGC1 beta peptide
Lane 9: Thymus (Rat) Tissue Lysate at 10 µg with PGC1 beta peptide
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 91 kDa
Observed band size: 110 kDa, 45 kDa, 73 kDa, 90 kDa
Exposure time: 8min
ELISA - Anti-PGC1 alpha + beta antibody (ab72230)
ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1μg/ml at 100μl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025μg/ml (100μl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.
Orange line: ELISA against immunising peptide ab73600
Yellow line: ELISA against ab88232
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