Anti-PGC1 alpha + beta antibody - BSA and Azide free

• WB

Unknown

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

False colour image of Western blot : Anti-PGC1 alpha + beta antibody - BSA and Azide free staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab54481 was shown to bind to PGC1 alpha and PGC1 beta. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Donkey anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216778) at 1/20000 dilution.

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481)

Lane 1:

Human PGC1 alpha recombinant (aa481-798) cell lysate at 0.1 µg

Lane 2:

Human PGC1 beta recombinant (aa550-1023 ) cell lysate at 0.1 µg

Lane 3:

Human skeletal muscle cell lysate at 20 µg

Lane 4:

Human heart cell lysate at 20 µg

Lane 5:

Human kidney cell lysate at 20 µg

Secondary

All lanes:

<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a> + <a href='/en-us/products/secondary-antibodies/donkey-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216778'>ab216778</a> at 1/20000 dilution

Predicted band size: 92 kDa

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• WB

Unknown

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

Western blot using ab54481 at 1 : 2000 to detect PGC1 in Mouse brown adipose tissue whole cell extract (both lanes).

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) at 1/2000 dilution

Lane 1:

Mouse Brown Adipose Tissue at 10 µg

Lane 2:

Mouse Brown Adipose Tissue at 20 µg

Predicted band size: 92 kDa

Observed band size: 105 kDa

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