AB54481

Anti-PGC1 alpha + beta antibody - BSA and Azide free

(26 Reviews)

(548 Publications)

Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) is a rabbit polyclonal antibody provided in a PBS only buffer for easy conjugation detecting PGC1 alpha + beta in Western Blot. Suitable for Human, Mouse.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 540 publications
- Trusted since 2007

View Alternative Names

peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta

2 Images

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Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

False colour image of Western blot : Anti-PGC1 alpha + beta antibody - BSA and Azide free staining at 1 μg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab54481 was shown to bind to PGC1 alpha and PGC1 beta. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Donkey anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216778) at 1/20000 dilution.

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481)

Lane 1:

Human PGC1 alpha recombinant (aa481-798) cell lysate at 0.1 µg

Lane 2:

Human PGC1 beta recombinant (aa550-1023 ) cell lysate at 0.1 µg

Lane 3:

Human skeletal muscle cell lysate at 20 µg

Lane 4:

Human heart cell lysate at 20 µg

Lane 5:

Human kidney cell lysate at 20 µg

Secondary

All lanes:

<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a> + <a href='/en-us/products/secondary-antibodies/donkey-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216778'>ab216778</a> at 1/20000 dilution

Predicted band size: 92 kDa

false

Unknown

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

Western blot using ab54481 at 1 : 2000 to detect PGC1 in Mouse brown adipose tissue whole cell extract (both lanes).

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) at 1/2000 dilution

Lane 1:

Mouse Brown Adipose Tissue at 10 µg

Lane 2:

Mouse Brown Adipose Tissue at 20 µg

Predicted band size: 92 kDa

Observed band size: 105 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human PPARGC1A aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBK2

Specificity

The immunogen sequence for this antibody was from PGC1 alpha, and is 78% homologous with PGC1 beta (Uniprot: Q86YN6), which this antibody also detects.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low." }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low." }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of PGC1 alpha + beta?
Anti-PGC1 alpha + beta - BSA and Azide free (ab54481) specifically detects a band for PGC1 alpha + beta (UniProt: Q9UBK2) at a molecular weight of 92kDa.

Trusted by the scientific community
Anti-PGC1 alpha + beta - BSA and Azide free (ab54481) was first used in a scientific publication in 2007 and has been cited over 540 times in peer-reviewed journals.

Reviewed by scientists
Anti-PGC1 alpha + beta - BSA and Azide free (ab54481) has over 20 independent reviews from customers.

This product may be covered by one or more of the following U.S. Patents.

6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGC1 alpha and beta also known as Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC-1α) and 1-beta (PGC-1β) are transcriptional coactivators with molecular weights of approximately 91 kDa and 102 kDa respectively. These proteins are important in regulating energy metabolism and are highly expressed in tissues with high energy demand such as skeletal muscle heart and brown adipose tissue. The proteins interact with nuclear hormone receptors and various transcription factors to control the expression of genes involved in energy homeostasis.

Biological function summary

PGC1 alpha and beta activate and coordinate the expression of genes involved in mitochondrial biogenesis and oxidative phosphorylation. They do not form a complex themselves but work with other transcriptional regulators to enhance gene expression. These proteins influence the metabolic processes of cells regulating the conversion of energy substrates into usable cellular energy. They are critical in adapting to different physiological conditions like exercise and fasting by modulating the capacity for energy production.

Pathways

PGC1 alpha and beta integrate signals from various pathways like the PPAR and AMPK pathways which are significant for energy balance and metabolism. PGC1 alpha particularly interacts with proteins such as NRF1 and NRF2 to promote mitochondrial function. Through these pathways PGC1 coactivators help regulate metabolism by altering the transcriptional activity of genes involved in energy expenditure and metabolic adaptation.

PGC1 alpha and beta have significant implications in conditions such as obesity and diabetes. Dysregulation of these proteins affects the balance of energy metabolism leading to metabolic disorders. PGC1 alpha in particular interacts with proteins like SIRT1 which influence insulin sensitivity and metabolic rate thereby playing a role in the development and progression of these diseases. The modulation of PGC1 activity has potential therapeutic implications for managing metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Acts as a key regulator of gluconeogenesis : stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed : 16753578, PubMed : 23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed : 23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).

See full target information PPARGC1A

Additional targets

PPARGC1B

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Publications (548)

Recent publications for all applications. Explore the full list and refine your search

BMC pharmacology & toxicology 24:82 PubMed38129888

2023

Amlodipine alleviates renal ischemia/reperfusion injury in rats through Nrf2/Sestrin2/PGC-1α/TFAM Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi Shirzad,Seyed Amin Mousavinezhad,Mohammad Panji,Moin Ala

Aging 15:11740-11763 PubMed37950725

2023

1,5-anhydro-D-fructose induces anti-aging effects on aging-associated brain diseases by increasing 5'-adenosine monophosphate-activated protein kinase activity via the peroxisome proliferator-activated receptor-γ co-activator-1α/brain-derived neurotrophic factor pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kiyoshi Kikuchi,Shotaro Otsuka,Seiya Takada,Kazuki Nakanishi,Kentaro Setoyama,Harutoshi Sakakima,Eiichiro Tanaka,Ikuro Maruyama

iScience 26:108278 PubMed38026227

2023

Short salsalate administration affects cell proliferation, metabolism, and inflammation in polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anish A Kanhai,Elena Sánchez-López,Thomas B Kuipers,Jan B van Klinken,Kyra L Dijkstra,Inge van der Veen,Hans J Baelde,Xuewen Song,York Pei,Hailiang Mei,Wouter N Leonhard,Oleg A Mayboroda,Dorien J M Peters

International journal of molecular sciences 24: PubMed37834400

2023

Diaphragm Fatigue in SMNΔ7 Mice and Its Molecular Determinants: An Underestimated Issue.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Cadile,Deborah Recchia,Massimiliano Ansaldo,Paola Rossi,Giorgia Rastelli,Simona Boncompagni,Lorenza Brocca,Maria Antonietta Pellegrino,Monica Canepari

Journal of nanobiotechnology 21:304 PubMed37644475

2023

Human milk extracellular vesicles enhance muscle growth and physical performance of immature mice associating with Akt/mTOR/p70s6k signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zitong Meng,Dong Zhou,Dan Lv,Quan Gan,Yuxiao Liao,Zhao Peng,Xiaolei Zhou,Shiyin Xu,Penglong Chi,Zhipeng Wang,Andreas K Nüssler,Xuefeng Yang,Liegang Liu,Dongrui Deng,Wei Yang

Scientific reports 13:12794 PubMed37550331

2023

Hepcidin deficiency in mice impairs white adipose tissue browning possibly due to a defect in de novo adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Christophe Deschemin,Céline Ransy,Frédéric Bouillaud,Soonkyu Chung,Bruno Galy,Carole Peyssonnaux,Sophie Vaulont

Antioxidants (Basel, Switzerland) 12: PubMed37627571

2023

Ginsenoside Rc, an Active Component of , Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Aeyung Kim,Sang-Min Park,No Soo Kim,Haeseung Lee

Journal of clinical and translational hepatology 11:1329-1340 PubMed37719964

2023

Proanthocyanidin Alleviates Liver Ischemia/Reperfusion Injury by Suppressing Autophagy and Apoptosis via the PPARα/PGC1α Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilu Yao,Ning Liu,Hui Lin,Yingqun Zhou

Molecular neurobiology 60:5102-5116 PubMed37256428

2023

Resveratrol Attenuates Chronic Unpredictable Mild Stress-Induced Alterations in the SIRT1/PGC1α/SIRT3 Pathway and Associated Mitochondrial Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sidra Tabassum,Afzal Misrani,Hui-Xian Huang,Zai-Yong Zhang,Qiao-Wei Li,Cheng Long

Basic research in cardiology 118:20 PubMed37212935

2023

SMYD1a protects the heart from ischemic injury by regulating OPA1-mediated cristae remodeling and supercomplex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta W Szulik,Steven Valdez,Maureen Walsh,Kathryn Davis,Ryan Bia,Emilee Horiuchi,Sean O'Very,Anil K Laxman,Linda Sandaklie-Nicolova,David R Eberhardt,Jessica R Durrant,Hanin Sheikh,Samuel Hickenlooper,Magnus Creed,Cameron Brady,Mickey Miller,Li Wang,June Garcia-Llana,Christopher Tracy,Stavros G Drakos,Katsuhiko Funai,Dipayan Chaudhuri,Sihem Boudina,Sarah Franklin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGC1 alpha + beta antibody - BSA and Azide free

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (AB54481)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (548)

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