Anti-PGC1 beta antibody [EPR12370]

6 product images

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  Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.
  

  Lane 1 (input): HeLa whole cell lysate (10μg)

  Lane 2 (+): ab176328 + HeLa whole cell lysate.

  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab176328 in HeLa whole cell lysate.

  For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

  All lanes: Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  Predicted band size: 113 kDa

  Observed band size: 113 kDa

• 

  Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  Blocking and dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution

  Lane 1: HeLa whole cell lysate at 20 µg

  Lane 2: MCF-7 whole cell lysate at 20 µg

  Lane 3: SH-SY5Y whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 113 kDa, 41 kDa

  Observed band size: 113 kDa, 40 kDa

• 

  Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.

  All lanes: Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  Predicted band size: 113 kDa

• 

  Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  Blocking and dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution

  Lane 1: Mouse spleen tissue lysate at 20 µg

  Lane 2: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 113 kDa

  Observed band size: 113 kDa

• 

  Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  All lanes: Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution

  Lane 1: HeLa cell lysate at 10 µg

  Lane 2: MCF7 cell lysate at 10 µg

  Lane 3: Human fetal brain tissue lysate at 10 µg

  Lane 4: Human fetal heart tissue lysate at 10 µg

  Predicted band size: 113 kDa

• 

  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)

  PGC1 beta western blot using anti-PGC1 beta antibody [EPR12370] ab176328. Publication image and figure legend from Lu, Y., Fujioka, H., et al., 2018, Sci Rep, PubMed 29844467.

  
  

  ab176328 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab176328 please see the product overview.

  Cold-activated BAT mitophagy coordinates with mitochondrial biogenesis and is critical for BAT mitochondrial homeostasis. (A) Western blots of key transcriptional activators for BAT mitochondrial biogenesis program PGC1α, PGC1β, TFAM, NRF1 and NRF2; mitochondrial proteins ATP5A, UCP1 and β-actin in BAT protein lysate from 7d 30 °C-acclimated (30 °C), 4 °C-challenged (4 °C), with or without CQ-treatment (30 °C + CQ and 4 °C + CQ) (n = 3 mice in each group). (C) Representative EM pictures of Veh or CQ-treated BAT mitochondria after 7d 4 °C-challenge (n = 3 mice per group). (D) Representative EM pictures of Becn+/− and control mice (n = 3 mice per group) BAT mitochondria after 7d 4 °C-challenge. (E) Representative EM pictures of Pink1−/− and WT mice (n = 3 mice per group) BAT mitochondria after 7d 4 °C-challenge. Mitophagosomes are marked by star, outlined by the red box and amplified in the inset. Damaged mitochondria are indicated by the red arrows. CQ: chloroquine; Veh: vehicle. Scale bars: 1 μm.