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Rabbit Recombinant Monoclonal PGD antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.

Alternative names

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Rabbit Recombinant Monoclonal PGD antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR6565

Purity

Tissue culture supernatant

Dissociation constant

4.53 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.

Activity summary

PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.

Pathways

PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.

Associated diseases and disorders

Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PGD antibody [EPR6565] (ab129199), expandable thumbnail

    Western blot - Anti-PGD antibody [EPR6565] (ab129199)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PGD antibody [EPR6565] (AB129199) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysates at 20 µg

    Lane 2: Mouse heart tissue lysates at 20 µg

    Lane 3: Mouse kidney tissue lysates at 20 µg

    Lane 4: Mouse spleen tissue lysates at 20 µg

    Lane 5: Rat brain tissue lysates at 20 µg

    Lane 6: Rat heart tissue lysates at 20 µg

    Lane 7: Rat kidney tissue lysates at 20 µg

    Lane 8: Rat spleen tissue lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 53 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

    This data was developed using ab129199, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (ab129199), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (ab129199)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with ab129199 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry (Intracellular) - Anti-PGD antibody [EPR6565] (ab129199), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGD antibody [EPR6565] (ab129199)

    Overlay histogram showing HEK293 cells stained with ab129199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129199, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-PGD antibody [EPR6565] (ab129199), expandable thumbnail

    Western blot - Anti-PGD antibody [EPR6565] (ab129199)

    All lanes: Western blot - Anti-PGD antibody [EPR6565] (AB129199) at 1/1000 dilution

    Lane 1: Raji cell lysate at 10 µg

    Lane 2: Molt-4 cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa

    Observed band size: 45 kDa

  • OI-RD Scanning - Anti-PGD antibody [EPR6565] (ab129199), expandable thumbnail

    OI-RD Scanning - Anti-PGD antibody [EPR6565] (ab129199)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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