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Rabbit Recombinant Monoclonal PGD antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (AB248347), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] - BSA and Azide free (AB248347), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PGD antibody [EPR6565] - BSA and Azide free (AB248347), expandable thumbnail
  • Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (AB248347), expandable thumbnail
  • OI-RD Scanning - Anti-PGD antibody [EPR6565] - BSA and Azide free (AB248347), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Expected
Rat
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.

Alternative names

PGDH, PGD

Recommended products

Rabbit Recombinant Monoclonal PGD antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6565
Purification technique
Affinity purification
Dissociation constant
4.53 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248347 is the carrier-free version of Anti-PGD antibody [EPR6565] ab129199.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.

Biological function summary

The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.

Pathways

PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.

Associated diseases and disorders

Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347), expandable thumbnail

    Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347)

    This data was developed using Anti-PGD antibody [EPR6565] ab129199, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PGD antibody [EPR6565] (Anti-PGD antibody [EPR6565] ab129199) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysates at 20 µg

    Lane 2: Mouse heart tissue lysates at 20 µg

    Lane 3: Mouse kidney tissue lysates at 20 µg

    Lane 4: Mouse spleen tissue lysates at 20 µg

    Lane 5: Rat brain tissue lysates at 20 µg

    Lane 6: Rat heart tissue lysates at 20 µg

    Lane 7: Rat kidney tissue lysates at 20 µg

    Lane 8: Rat spleen tissue lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347)

    This data was developed using Anti-PGD antibody [EPR6565] ab129199, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with Anti-PGD antibody [EPR6565] ab129199 at 1/500. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry (Intracellular) - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347)

    This data was developed using Anti-PGD antibody [EPR6565] ab129199, the same antibody clone in a different buffer formulation.Overlay histogram showing HEK293 cells stained with Anti-PGD antibody [EPR6565] ab129199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-PGD antibody [EPR6565] ab129199, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347), expandable thumbnail

    Western blot - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347)

    This data was developed using Anti-PGD antibody [EPR6565] ab129199, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PGD antibody [EPR6565] (Anti-PGD antibody [EPR6565] ab129199) at 1/1000 dilution

    Lane 1: Raji cell lysate at 10 µg

    Lane 2: Molt-4 cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa

    Observed band size: 45 kDa

  • OI-RD Scanning - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347), expandable thumbnail

    OI-RD Scanning - Anti-PGD antibody [EPR6565] - BSA and Azide free (ab248347)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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