AB113687

Anti-PGK1 antibody [22C5D8]

Name: Anti-PGK1 antibody [22C5D8] (ab113687) | Abcam
Brand: Abcam Limited
SKU: AB113687
Price: 415 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(188 Publications)

Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody detecting PGK1 in Western Blot. Suitable for Saccharomyces cerevisiae.

- Over 140 publications

View Alternative Names

YCR012W, YCR12W, PGK1, Phosphoglycerate kinase

2 Images

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Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

All lanes:

Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)

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Yeast whole cell lysate at 15 µg

Predicted band size: 45 kDa

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AbReview60407****

Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration : 5% · Temperature : 25°C.

Gel running condition : Reduced Denaturing (4-12% Bis-Tris).

All lanes:

Western blot - Anti-PGK1 antibody [22C5D8] (ab113687) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae Cell lysate - nuclear at 30 µg

Secondary

All lanes:

Sheep anti-Mouse IgG, HRP-linked whole Ab ECL at 1/5000 dilution

Predicted band size: 45 kDa

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Exposure time: 1min

This image is courtesy of an anonymous abreview.

HRP

HRP Anti-PGK1 antibody [22C5D8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

22C5D8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Saccharomyces cerevisiae samples.

What is the molecular weight of PGK1?
Anti-PGK1 [22C5D8] (ab113687) specifically detects a band for PGK1 (UniProt: P00558) at a molecular weight of 45kDa.

Trusted by the scientific community
Anti-PGK1 [22C5D8] (ab113687) was first used in a scientific publication in 2011 and has been cited over 140 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [22C5D8] also available for your convenience: ab113687, HRP - ab197960

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.

Biological function summary

The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.

Pathways

The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.

PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 6254992). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (By similarity). Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion (By similarity).

See full target information PGK1

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Publications (188)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7212 PubMed40764615

2025

Arp2/3 and type-I myosins control chromosome mobility and end-resection at double-strand breaks in S. cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Y Zhou,Marissa Ashton,Yiyang Jiang,Neha Arora,Kevin Clark,Kate B Fitzpatrick,James E Haber

Nature communications 16:5314 PubMed40595511

2025

A constricted mitochondrial morphology formed during respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Manish K Singh,Laetitia Cavellini,Maria Angeles Morcillo-Parra,Christina Kunz,Mickaël Lelek,Perrine Bomme,Aurélia Barascu,Cynthia Alsayyah,Maria Teresa Teixeira,Naïma Belgareh-Touzé,Adeline Mallet,Lea Dietrich,Christophe Zimmer,Mickael M Cohen

Protein science : a publication of the Protein Society 34:e70207 PubMed40545685

2025

Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Friederike M C Benning,Tristan A Bell,Tran H Nguyen,Della Syau,Louise B Connell,Yi-Ting Liao,Matthew P Keating,Margaret Coughlin,Anja E H Nordstrom,Maria Ericsson,Corrie J B daCosta,Luke H Chao

Genetics 231: PubMed40546018

2025

Revealing function-altering MECP2 mutations in individuals with autism spectrum disorder using yeast and Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Chen,Jessica Schmitt,Graeme McIntosh,Barry P Young,Tianshun Lian,Jie Liu,Kexin K Chen,J Beatrice Liston,Lily MacDonald,Bill Wang,Sonia Medina Giro,Benjamin Boehme,Mriga Das,Seevasant Indran,Jesse T Chao,Sanja Rogic,Paul Pavlidis,Douglas W Allan,Christopher J R Loewen

Life science alliance 8: PubMed40541417

2025

Hydroxyurea modulates thiol-disulfide homeostasis in the yeast endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Takano,Yuki Ishiwata-Kimata,Ryo Ushioda,Yukio Kimata,Kunio Nakatsukasa

microPublication biology 2025: PubMed40406580

2025

Pep12 is important for proteasome microautophagy under low glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle VanderVen,Conner Butcher,Remi' Fokine,Jianhui Li

Frontiers in cell and developmental biology 13:1554958 PubMed40365021

2025

Omic AI reveals new autophagy regulators from the Atg1 interactome in .

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Han,Shanshan Fu,Dachao Tang,Yuting Chen,Dan Liu,Zihao Feng,Yujie Gou,Chi Zhang,Weizhi Zhang,Leming Xiao,Jiayi Zhang,Cong Yi,Yu Xue,Di Peng

PLoS biology 23:e3003111 PubMed40208903

2025

Phase separation of the PRPP amidotransferase into dynamic condensates promotes de novo purine synthesis in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Masak Takaine,Rikuri Morita,Yuto Yoshinari,Takashi Nishimura

Genetics 229: PubMed40178993

2025

A new set of mutations in the second transmembrane helix of the Cox2p-W56R substantially improves its allotopic expression in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Kewin Gombeau,Stefan A Hoffmann,Yizhi Cai

Nature structural & molecular biology 32:1006-1018 PubMed39930008

2025

Initiation of ERAD by the bifunctional complex of Mnl1/Htm1 mannosidase and protein disulfide isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhao,Xudong Wu,Tom A Rapoport

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGK1 antibody [22C5D8]

Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (188)

Product promise