Anti-PGK1 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PGK1 antibody (AB154613)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PGK1 using ab154613 at 1/50 dilution. Right panel costained with Hoechst 33342.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGK1 antibody (AB154613)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling PGK1 protein at cytoplasm and nucleus using ab154613 at a 1/20 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/10000 dilution (Red).
Hoechst 33342 was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/2000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Predicted band size: 45 kDa

false

• WB

Unknown

Western blot - Anti-PGK1 antibody (AB154613)

10% SDS PAGE

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate (untreated) at 30 µg

Lane 2:

HeLa whole cell lysate (1% O2 treatment for 24hr) at 30 µg

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell lysates at 30 µg

Lane 2:

PGK1 shRNA transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/1000 dilution

All lanes:

Rat liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

true

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/3000 dilution

Lane 1:

Non-transfected 293T whole cell lysates at 30 µg

Lane 2:

PGK1-transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/500 dilution

Lane 1:

Saccharomyces cerevisiae (S288C) extracts at 30 µg

Lane 2:

Saccharomyces cerevisiae (W303-1a) extracts at 30 µg

Lane 3:

Saccharomyces cerevisiae (W303-1b) extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

true

• WB

Supplier Data

Western blot - Anti-PGK1 antibody (AB154613)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 antibody (ab154613) at 1/1000 dilution

All lanes:

Yeast (S. cerevisiae) whole lysates

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false