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AB305130

Anti-PGK1 antibody [EPR24614-29] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal PGK1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2

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Sandwich ELISA - Anti-PGK1 antibody [EPR24614-29] - BSA and Azide free (Capture) (AB305130)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PGK1 antibody [EPR24614-29] - BSA and Azide free (Capture) (AB305130)

Sandwich ELISA of ab305079 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24614-29

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
Biological function summary

The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.

Pathways

The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.

PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 30323285, PubMed : 7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed : 18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed : 2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed : 26942675, PubMed : 36849569). May play a role in sperm motility (PubMed : 26677959).
See full target information PGK1
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Product promise

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