Skip to main content

Rabbit Recombinant Monoclonal PGK1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (AB186742), expandable thumbnail
  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (AB186742), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (AB186742), expandable thumbnail
  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (AB186742), expandable thumbnail
  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (AB186742), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).

Additional Targets

PGK2

Alternative names

PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2

Recommended products

Rabbit Recombinant Monoclonal PGK1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14908
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Phosphoglycerate kinase 1 (PGK1) and phosphoglycerate kinase 2 (PGK2) are enzymes involved in glycolytic processes. PGK1 also known as PGKA has a molecular mass of approximately 45 kDa and is widely expressed in most tissues especially in those requiring high energy. PGK2 alternatively known as testis-specific PGK has a similar molecular mass of about 45 kDa and is mainly expressed in the testis. Both enzymes ensure the important step of catalyzing the transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP.

Biological function summary

Phosphoglycerate kinase 1 and 2 are important for cellular energy production and metabolic homeostasis. PGK1 plays a role in maintaining general cellular energy needs especially in environments with fluctuating oxygen levels. PGK2 as a testis-specific isoform supports spermatogenesis by facilitating glycolysis important for sperm motility. Both enzymes do not form part of a larger enzymatic complex functioning as standalone entities in their respective pathways.

Pathways

Phosphoglycerate kinases participate in the glycolytic pathway which is essential for glucose catabolism and ATP generation. Within glycolysis PGK1 and PGK2 work with other glycolytic enzymes such as hexokinase and pyruvate kinase. Both enzymes help balance cellular redox states through their action in redox and energy production pathways intimately linked to other metabolic states such as those found in oxidative phosphorylation.

Associated diseases and disorders

Overactivity of PGK1 has associations with cancer where altered glycolytic activity supports rapid tumor growth. Its partner proteins like HIF-1α help mediate these changes in cancer metabolism. Additionally PGK2 deficiency can result in male infertility due to its indispensable role in sperm energy metabolism. There is no prominent disease linked partner for PGK2 outside its glycolytic function within testis tissue.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742), expandable thumbnail

    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    All lanes: Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/20000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: Human testis cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742), expandable thumbnail

    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    All lanes: Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: Raw264.7 cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

  • Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells labeling PGK1 with ab186742 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742), expandable thumbnail

    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    Blocking and Diluting Buffer: 5% NFDM /TBST

    Lanes 3 and 4 Exposure: 60 seconds

    Lanes 1 - 2: Western blot - Anti-PGK1 antibody [EPR19057] (Anti-PGK1 antibody [EPR19057] ab199438) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

    Lanes 1 and 3: PGK1 Human Recombinant Protein at 20 µg

    Lanes 2 and 4: PGK2 Human Recombinant Protein at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa

    Exposure time: 5s

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742), expandable thumbnail

    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    Blocking and Diluting Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

    Lane 1: PGK1 Human Recombinant Protein at 20 µg

    Lane 2: PGK2 Human Recombinant Protein at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa

    Exposure time: 60s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com