AB308196

Anti-PGK1 + PGK2 antibody [HL1775]

Recombinant
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Rabbit Recombinant Monoclonal PGK1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human PGK1.

View Alternative Names

PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2

5 Images

Immunohistochemistry paraffin embedded sections - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Immunohistochemical analysis of paraffin-embedded mouse testis labeling PGK1 + PGK2 protein with ab308196 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Immunohistochemical analysis of paraffin-embedded rat testis labeling PGK1 + PGK2 protein with ab308196 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (ab308196) diluted at 1/10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (ab308196) at 1/10000 dilution

Lane 1:

HEK-293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.6 kDa

false

Supplier Data

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (ab308196) diluted at 1/10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (ab308196) at 1/10000 dilution

Lane 1:

Mouse liver tissue lysate at 50 µg

Lane 2:

Rat liver tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.6 kDa

false

Supplier Data

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (ab308196) at 1/5000 dilution

All lanes:

Whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.6 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1775

Isotype

IgG

Carrier free

Yes

Reacts with

Zebrafish, Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PGK1. The exact immunogen used to generate this antibody is proprietary information.

P00558

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate kinase 1 (PGK1) and phosphoglycerate kinase 2 (PGK2) are enzymes involved in glycolytic processes. PGK1 also known as PGKA has a molecular mass of approximately 45 kDa and is widely expressed in most tissues especially in those requiring high energy. PGK2 alternatively known as testis-specific PGK has a similar molecular mass of about 45 kDa and is mainly expressed in the testis. Both enzymes ensure the important step of catalyzing the transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP.

Biological function summary

Phosphoglycerate kinase 1 and 2 are important for cellular energy production and metabolic homeostasis. PGK1 plays a role in maintaining general cellular energy needs especially in environments with fluctuating oxygen levels. PGK2 as a testis-specific isoform supports spermatogenesis by facilitating glycolysis important for sperm motility. Both enzymes do not form part of a larger enzymatic complex functioning as standalone entities in their respective pathways.

Pathways

Phosphoglycerate kinases participate in the glycolytic pathway which is essential for glucose catabolism and ATP generation. Within glycolysis PGK1 and PGK2 work with other glycolytic enzymes such as hexokinase and pyruvate kinase. Both enzymes help balance cellular redox states through their action in redox and energy production pathways intimately linked to other metabolic states such as those found in oxidative phosphorylation.

Overactivity of PGK1 has associations with cancer where altered glycolytic activity supports rapid tumor growth. Its partner proteins like HIF-1α help mediate these changes in cancer metabolism. Additionally PGK2 deficiency can result in male infertility due to its indispensable role in sperm energy metabolism. There is no prominent disease linked partner for PGK2 outside its glycolytic function within testis tissue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 30323285, PubMed : 7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed : 18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed : 2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed : 26942675, PubMed : 36849569). May play a role in sperm motility (PubMed : 26677959).

See full target information PGK1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGK1 + PGK2 antibody [HL1775]

Immunohistochemistry paraffin embedded sections - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Immunohistochemistry paraffin embedded sections - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Western blot - Anti-PGK1 + PGK2 antibody [HL1775] (AB308196)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise