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AB183031

Anti-PGK2 antibody [EPR14909(B)]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal PGK2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 7 publications.

View Alternative Names

PGKB, PGK2, Phosphoglycerate kinase 2

4 Images
Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
  • WB

Supplier Data

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)

All lanes:

Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 20 µg

Lane 2:

Rat testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
  • WB

Supplier Data

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)

All lanes:

Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human seminoma lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
  • WB

Lab

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)

Blocking and diluting buffer : 5% NFDM /TBST

Lanes 1 - 2:

Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/pgk2-antibody-epr14909b-bsa-and-azide-free-ab236139'>ab236139</a>)

Lane 1:

PGK1 Human Recombinant Protein at 20 µg

Lane 2:

PGK2 Human Recombinant Protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
  • WB

CiteAb

Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)

PGK2 western blot using anti-PGK2 antibody [EPR14909(B)] ab183031. Publication image and figure legend from Wu, S. T., Liu, B., et al., 2020, Front Pharmacol, PubMed 32292350.

ab183031 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183031 please see the product overview.

(A) Effects of esculetin on final tumor weight. (B) Comparison of PGK2, GPD2, GPI, Casepase-3, BAX, Bcl-2, and GAPDH levels in tumor tissue from different groups by WB, 0 mg/kg group was used as a control. Results are means± SEM, n=3 per experiment, *p < 0.05, **p < 0.01.

false

  • Carrier free

    Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14909(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate kinase 2 (PGK2) also known as testis-specific phosphoglycerate kinase is an enzyme with a molecular mass of approximately 45 kDa. It functions in glycolysis by catalyzing the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate producing ATP in the process. PGK2 expression is restricted mostly to the testes where it plays an important role in sperm development and motility. Unlike its isozyme counterpart PGK1 which is expressed ubiquitously PGK2 is testis-specific.
Biological function summary

The enzyme participates in the important processes of energy production and spermatozoa development. While PGK2 does not form part of any multi-protein complex it is an integral component of the glycolytic pathway contributing to ATP production necessary for sperm motility. Its expression is regulated during spermatogenesis highlighting its role in male fertility.

Pathways

PGK2 operates within the glycolytic pathway a major metabolic route for energy production. Along with glyceraldehyde-3-phosphate dehydrogenase PGK2 catalyzes important reactions that facilitate the conversion of glucose to pyruvate yielding high-energy ATP molecules. By functioning in tandem with other glycolytic enzymes PGK2 ensures the efficient production of energy needed for cellular functions especially during spermatogenesis.

Defects or alterations in PGK2 have associations with male infertility notably due to impaired sperm function. Disorders like spermatogenic failure and related conditions arise when PGK2 activity diminishes. Its enzyme partner glyceraldehyde-3-phosphate dehydrogenase-S is also linked to sperm motility issues. Understanding PGK2’s role aids in identifying therapeutic targets to address such reproductive challenges.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and plays a role in glycolysis and gluconeogenesis (By similarity). Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).
See full target information PGK2

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5113 PubMed37607933

2023

SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Liu,Louis Hermo,Deqiang Ding,Chao Wei,Jeffrey M Mann,Xiaoyuan Yan,Ashley F Melnick,Yingjie Wu,Alicia Withrow,Jose Cibelli,Rex A Hess,Chen Chen

Pharmaceutical biology 60:319-325 PubMed35148224

2022

Peptides from the croceine croaker () swim bladder attenuate busulfan-induced oligoasthenospermia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhao,Mengmeng Sang,Ping Han,Jie Gao,Zhenhua Liu,Hu Li,Yayun Gu,Chengniu Wang,Fei Sun

Frontiers in oncology 11:644492 PubMed34221965

2021

The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Chen,Weiqiang Jia,Cuiying Wu,Lihua Chen,Kai Sun,Ji Wang,Boyun Ding,Ning Liu,Ruxiang Xu

Molecular medicine reports 24: PubMed34109434

2021

Shikonin inhibits the Warburg effect, cell proliferation, invasion and migration by downregulating PFKFB2 expression in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Sha,Zhiqiang Lv,Yujun Liu,Yun Zhang,Xin Sui,Teng Wang,Hui Zhang

Journal of cellular and molecular medicine 25:1624-1632 PubMed33438283

2021

Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexia Liu,Qian Li,Zhixin Wang,Fujun Liu

Frontiers in pharmacology 11:379 PubMed32292350

2020

Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Tao Wu,Bo Liu,Zhong-Zhu Ai,Zong-Chao Hong,Peng-Tao You,He-Zhen Wu,Yan-Fang Yang

Molecular medicine reports 19:2471-2478 PubMed30720094

2019

Aberrant expression of sperm‑specific glycolytic enzymes are associated with poor sperm quality.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexia Liu,Qian Li,Wenjuan Wang,Fujun Liu
View all publications

Product promise

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