Rabbit Recombinant Monoclonal PGK2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 7 publications.
View Alternative Names
PGKB, PGK2, Phosphoglycerate kinase 2
- WB
Supplier Data
Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
All lanes:
Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lane 1:
Mouse testis lysate at 20 µg
Lane 2:
Rat testis lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
false
- WB
Supplier Data
Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
All lanes:
Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lane 1:
Human testis lysate at 10 µg
Lane 2:
Human seminoma lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
false
- WB
Lab
Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
Blocking and diluting buffer : 5% NFDM /TBST
Lanes 1 - 2:
Western blot - Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lanes 1 - 2:
Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/pgk2-antibody-epr14909b-bsa-and-azide-free-ab236139'>ab236139</a>)
Lane 1:
PGK1 Human Recombinant Protein at 20 µg
Lane 2:
PGK2 Human Recombinant Protein at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 45 kDa
false
- WB
CiteAb
Western blot - Anti-PGK2 antibody [EPR14909(B)] (AB183031)
PGK2 western blot using anti-PGK2 antibody [EPR14909(B)] ab183031. Publication image and figure legend from Wu, S. T., Liu, B., et al., 2020, Front Pharmacol, PubMed 32292350.
ab183031 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183031 please see the product overview.
(A) Effects of esculetin on final tumor weight. (B) Comparison of PGK2, GPD2, GPI, Casepase-3, BAX, Bcl-2, and GAPDH levels in tumor tissue from different groups by WB, 0 mg/kg group was used as a control. Results are means± SEM, n=3 per experiment, *p < 0.05, **p < 0.01.
false
Related conjugates and formulations (1)
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Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme participates in the important processes of energy production and spermatozoa development. While PGK2 does not form part of any multi-protein complex it is an integral component of the glycolytic pathway contributing to ATP production necessary for sperm motility. Its expression is regulated during spermatogenesis highlighting its role in male fertility.
Pathways
PGK2 operates within the glycolytic pathway a major metabolic route for energy production. Along with glyceraldehyde-3-phosphate dehydrogenase PGK2 catalyzes important reactions that facilitate the conversion of glucose to pyruvate yielding high-energy ATP molecules. By functioning in tandem with other glycolytic enzymes PGK2 ensures the efficient production of energy needed for cellular functions especially during spermatogenesis.
Product protocols
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Target data
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 14:5113 PubMed37607933
2023
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Pharmaceutical biology 60:319-325 PubMed35148224
2022
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Unspecified reactive species
Frontiers in oncology 11:644492 PubMed34221965
2021
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Unspecified reactive species
Molecular medicine reports 24: PubMed34109434
2021
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Unspecified application
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Unspecified reactive species
Journal of cellular and molecular medicine 25:1624-1632 PubMed33438283
2021
Applications
Unspecified application
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Unspecified reactive species
Frontiers in pharmacology 11:379 PubMed32292350
2020
Applications
Unspecified application
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Unspecified reactive species
Molecular medicine reports 19:2471-2478 PubMed30720094
2019
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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