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AB236139

Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PGK2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

PGKB, PGK2, Phosphoglycerate kinase 2

2 Images
Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (AB236139)
  • WB

Supplier Data

Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (AB236139)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183031).

All lanes:

Western blot - Anti-PGK2 antibody [EPR14909(B)] (<a href='/en-us/products/primary-antibodies/pgk2-antibody-epr14909b-ab183031'>ab183031</a>) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 20 µg

Lane 2:

Rat testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (AB236139)
  • WB

Lab

Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (AB236139)

Blocking and diluting buffer : 5% NFDM /TBST This data was developed using the same antibody clone in a different buffer formulation (ab183031).

Lanes 1 - 2:

Western blot - Anti-PGK2 antibody [EPR14909(B)] (<a href='/en-us/products/primary-antibodies/pgk2-antibody-epr14909b-ab183031'>ab183031</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-PGK2 antibody [EPR14909(B)] - BSA and Azide free (ab236139)

Lane 1:

PGK1 Human Recombinant Protein at 20 µg

Lane 2:

PGK2 Human Recombinant Protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14909(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab236139 is the carrier-free version of ab183031.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate kinase 2 (PGK2) also known as testis-specific phosphoglycerate kinase is an enzyme with a molecular mass of approximately 45 kDa. It functions in glycolysis by catalyzing the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate producing ATP in the process. PGK2 expression is restricted mostly to the testes where it plays an important role in sperm development and motility. Unlike its isozyme counterpart PGK1 which is expressed ubiquitously PGK2 is testis-specific.
Biological function summary

The enzyme participates in the important processes of energy production and spermatozoa development. While PGK2 does not form part of any multi-protein complex it is an integral component of the glycolytic pathway contributing to ATP production necessary for sperm motility. Its expression is regulated during spermatogenesis highlighting its role in male fertility.

Pathways

PGK2 operates within the glycolytic pathway a major metabolic route for energy production. Along with glyceraldehyde-3-phosphate dehydrogenase PGK2 catalyzes important reactions that facilitate the conversion of glucose to pyruvate yielding high-energy ATP molecules. By functioning in tandem with other glycolytic enzymes PGK2 ensures the efficient production of energy needed for cellular functions especially during spermatogenesis.

Defects or alterations in PGK2 have associations with male infertility notably due to impaired sperm function. Disorders like spermatogenic failure and related conditions arise when PGK2 activity diminishes. Its enzyme partner glyceraldehyde-3-phosphate dehydrogenase-S is also linked to sperm motility issues. Understanding PGK2’s role aids in identifying therapeutic targets to address such reproductive challenges.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).
See full target information PGK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32163370

2020

ATR expands embryonic stem cell fate potential in response to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Atashpaz,Sara Samadi Shams,Javier Martin Gonzalez,Endre Sebestyén,Negar Arghavanifard,Andrea Gnocchi,Eliene Albers,Simone Minardi,Giovanni Faga,Paolo Soffientini,Elisa Allievi,Valeria Cancila,Angela Bachi,Óscar Fernández-Capetillo,Claudio Tripodo,Francesco Ferrari,Andrés Joaquin López-Contreras,Vincenzo Costanzo
View all publications
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Product promise

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