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AB229872

Anti-PGLS antibody

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(2 Publications)

Rabbit Polyclonal PGLS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGLS.

View Alternative Names

6-phosphogluconolactonase, 6PGL, PGLS

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (AB229872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (AB229872)

Paraffin-embedded human thyroid tissue stained for PGLS with ab229872 at a 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (AB229872)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (AB229872)

Paraffin-embedded human tonsil tissue stained for PGLS with ab229872 at a 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-PGLS antibody (AB229872)
  • IP

Supplier Data

Immunoprecipitation - Anti-PGLS antibody (AB229872)

PGLS was immunoprecipitated from 500 μg U251 whole cell extracts using 4 μg of ab229872.

Lane 1 : Control IgG IP in U251 whole cell extracts.

Lane 2 : ab229872 in U251 whole cell extracts.

Lane 3 : PGLS in U251 whole cell extracts (Input).

For WB an HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000).

All lanes:

Immunoprecipitation - Anti-PGLS antibody (ab229872)

Predicted band size: 28 kDa

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Western blot - Anti-PGLS antibody (AB229872)
  • WB

Supplier Data

Western blot - Anti-PGLS antibody (AB229872)

All lanes:

Western blot - Anti-PGLS antibody (ab229872) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse brain tissue lysate

Lane 4:

U251 whole cell lysate

Lane 5:

THP-1 (Human monocytic leukemia cell line) whole cell lysate

Lane 6:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 7:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PGLS.

O95336

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGLS also known as 6-phosphogluconolactonase operates in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone into 6-phosphogluconate. This enzyme has an approximate mass of 27 kDa. PGLS expresses widely in various tissues but it is especially abundant in liver and adipose tissues where the pentose phosphate pathway activity is high.
Biological function summary

6-phosphogluconolactonase plays a role in cellular metabolism by ensuring the smooth progression of the pentose phosphate pathway. This pathway is important for the production of NADPH which acts as a reducing agent in several biosynthetic reactions. PGLS does not form part of a multiprotein complex but serves a singular enzymatic role essential for maintaining cellular redox state.

Pathways

The pentose phosphate pathway lies at the center of PGLS's function in its role of maintaining NADPH levels for reductive biosynthesis and protection against oxidative stress. In this pathway PGLS closely associates with glucose-6-phosphate dehydrogenase (G6PD) which provides the substrate for PGLS. Both play a sequential role in the oxidative branch of the pentose phosphate pathway influencing cellular processes such as fatty acid synthesis and ribose-5-phosphate production.

PGLS connects to the metabolic and oxidative balance within cells indirectly affecting conditions like cancer and metabolic disorders. Alterations in its activity or expression could lead to dysregulation of NADPH levels linking it with oxidative stress-related pathologies. PGLS's functional relation to G6PD could influence its involvement in diseases where altered glucose metabolism and the oxidative state are disturbed.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
See full target information PGLS
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular biotechnology 67:689-704 PubMed38456959

2024

O-sialoglycoprotein Endopeptidase (OSGEP) Suppresses Hepatic Ischemia-Reperfusion Injury-Induced Ferroptosis Through Modulating the MEK/ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Tao,Wanqing Zhou,Cheng Chen,Qian Zhang,Zhuoyi Liu,Pingping Xia,Zhi Ye,Chunling Li

Cell death discovery 8:59 PubMed35149670

2022

YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao Zhang
View all publications
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