Rabbit Polyclonal PGLS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGLS.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PGLS. Database link O95336
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
Alternative names
6-phosphogluconolactonase, 6PGL, PGLS
Recommended products
Rabbit Polyclonal PGLS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGLS.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PGLS. Database link O95336
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGLS also known as 6-phosphogluconolactonase operates in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone into 6-phosphogluconate. This enzyme has an approximate mass of 27 kDa. PGLS expresses widely in various tissues but it is especially abundant in liver and adipose tissues where the pentose phosphate pathway activity is high.
- Biological function summary
6-phosphogluconolactonase plays a role in cellular metabolism by ensuring the smooth progression of the pentose phosphate pathway. This pathway is important for the production of NADPH which acts as a reducing agent in several biosynthetic reactions. PGLS does not form part of a multiprotein complex but serves a singular enzymatic role essential for maintaining cellular redox state.
- Pathways
The pentose phosphate pathway lies at the center of PGLS's function in its role of maintaining NADPH levels for reductive biosynthesis and protection against oxidative stress. In this pathway PGLS closely associates with glucose-6-phosphate dehydrogenase (G6PD) which provides the substrate for PGLS. Both play a sequential role in the oxidative branch of the pentose phosphate pathway influencing cellular processes such as fatty acid synthesis and ribose-5-phosphate production.
- Associated diseases and disorders
PGLS connects to the metabolic and oxidative balance within cells indirectly affecting conditions like cancer and metabolic disorders. Alterations in its activity or expression could lead to dysregulation of NADPH levels linking it with oxidative stress-related pathologies. PGLS's functional relation to G6PD could influence its involvement in diseases where altered glucose metabolism and the oxidative state are disturbed.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-PGLS antibody (ab229980)
All lanes: Western blot - Anti-PGLS antibody (ab229980) at 1/1000 dilution
Lane 1: Mouse liver lysate
Lane 2: Mouse brain lysate
Lane 3: U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4: THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 5: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa, 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229980)
Paraffin-embedded human thyroid tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-PGLS antibody (ab229980)
PGLS was immunoprecipitated from 500 μg U-251 MG (human brain glioma cell line) whole cell lysate with 4 μg ab229980.
Lane 1: Rabbit monoclonal IgG (1 μg) IP in U-251 MG whole cell lysate.
Lane 2: ab229980 IP in U-251 MG whole cell lysate.
Lane 3: U-251 MG whole cell lysate 20 μg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
All lanes: Immunoprecipitation - Anti-PGLS antibody (ab229980)
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGLS antibody (ab229980)
Paraffin-embedded human tonsil tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
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