AB223469

Anti-PGM1 antibody [CL3299]

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(3 Publications)

Mouse Monoclonal PGM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGM1 aa 400-500.

View Alternative Names

Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Paraffin embedded human prostate stained for PGM1 with ab223469 at a 1/2500 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Paraffin embedded human liver stained for PGM1 with ab223469 at a 1/2500 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Paraffin embedded human skeletal muscle stained for PGM1 with ab223469 at a 1/2500 dilution in Immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Paraffin embedded human colon stained for PGM1 with ab223469 at a 1/2500 dilution in Immunohistochemical analysis.

Lab

Western blot - Anti-PGM1 antibody [CL3299] (AB223469)

Western blot : Anti-PGM1 antibody [CL3299] ab223469 staining at 1/500 dilution, shown in green; Rabbit anti GAPDH ab181602 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 68 kDa in Wild-type A549 cell lysates with no signal observed at this size in PGM1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PGM1 antibody [CL3299] (ab223469) at 1/500 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PGM1 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

K562 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 68 kDa,37 kDa

false

Supplier Data

Western blot - Anti-PGM1 antibody [CL3299] (AB223469)

All lanes:

Western blot - Anti-PGM1 antibody [CL3299] (ab223469) at 1/500 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 61 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3299

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PGM1 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P36871

Epitope

Binds to an epitope located within the peptide sequence HGRNFFTRYD as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Protein PGM1 also known as phosphoglucomutase-1 plays a significant mechanical role in cellular glucose metabolism. It catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate a critical step in the glycolysis and gluconeogenesis pathways. The PGM1 protein has a molecular mass of approximately 61 kDa. Its expression occurs in various tissues with high levels in muscle and liver tissues which are key sites for energy storage and utilization.

Biological function summary

PGM1 contributes significantly to both energy homeostasis and carbohydrate metabolism. It functions as part of a dynamic complex involving other enzymes that manage intracellular energy balance. PGM1 supports glycogen synthesis and breakdown by regulating the availability of glucose-1-phosphate. This dual role makes PGM1 an important protein for maintaining normal metabolic function. Its activity impacts cellular energy levels which is vital for muscle contraction and overall energy distribution in tissues.

Pathways

PGM1 is critically involved in carbohydrate metabolic pathways specifically glycolysis and gluconeogenesis. In glycolysis it participates in converting glucose for energy production. In gluconeogenesis it assists in the generation of glucose from non-carbohydrate substrates. PGM1 interacts closely with other enzymes in these pathways such as glucose-6-phosphate dehydrogenase ensuring efficient energy regulation and metabolic flow within the cell.

PGM1 is associated with glycogen storage disease type XIV which is a condition causing improper glycogen metabolism. Mutations in the PGM1 gene disrupt its enzymatic function leading to symptoms like muscle pain and weakness. Additionally PGM1 defects can impact another protein phosphoglucomutase-3 (PGM3) which shares pathway similarities and potentially affects the synthesis of other phosphorylated sugars related to immune function. These connections highlight the importance of functional PGM1 in maintaining normal physiological and disease-free states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate (PubMed : 15378030, PubMed : 25288802). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable) (PubMed : 25288802). This enzyme participates in both the breakdown and synthesis of glucose (PubMed : 17924679, PubMed : 25288802).

See full target information PGM1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40724844

2025

HER2-Driven Breast Cancer: Role of the Chaperonin HSP90 in Modulating Response to Trastuzumab-Based Therapeutic Combinations.

Applications

Unspecified application

Species

Unspecified reactive species

Italia Falcone,Elena Giontella,Stefano Giuliani,Giulia Borghesani,Alessandro Valenti,Valentina Zambonin,Sara Monteverdi,Luisa Carbognin,Emilio Bria,Ludovica Ciuffreda,Fabiana Conciatori,Chiara Bazzichetto,Serena Pedron,Alessia Nottegar,Sara Zanelli,Alice Muzzarelli,Alessandra Fabi,Silvia Migliaccio,Elisabetta Ferretti,Roberto Bei,Elena Fiorio,Maurizio Fanciulli,Isabella Sperduti,Anna Caliò,Michele Milella

Journal of animal science 97:2837-2849 PubMed31267132

2019

Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiushi Xu,Xiaobing Li,Li Ma,Juan J Loor,Danielle N Coleman,Hongdou Jia,Guowen Liu,Chuang Xu,Yazhe Wang,Xinwei Li

Journal of animal science : PubMed30985866

2019

Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiushi Xu,Xiaobing Li,Li Ma,Juan J Loor,Danielle N Coleman,Hongdou Jia,Guowen Liu,Chuang Xu,Yazhe Wang,Xinwei Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGM1 antibody [CL3299]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM1 antibody [CL3299] (AB223469)

Western blot - Anti-PGM1 antibody [CL3299] (AB223469)

Western blot - Anti-PGM1 antibody [CL3299] (AB223469)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise