AB250979

Anti-PGM1 antibody [EPR15240] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PGM1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1

4 Images

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

This data was developed using ab188869, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Unknown

Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

This data was developed using ab188869, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

This data was developed using ab188869, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

This data was developed using ab188869, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15240

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250979 is the carrier-free version of ab188869.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Protein PGM1 also known as phosphoglucomutase-1 plays a significant mechanical role in cellular glucose metabolism. It catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate a critical step in the glycolysis and gluconeogenesis pathways. The PGM1 protein has a molecular mass of approximately 61 kDa. Its expression occurs in various tissues with high levels in muscle and liver tissues which are key sites for energy storage and utilization.

Biological function summary

PGM1 contributes significantly to both energy homeostasis and carbohydrate metabolism. It functions as part of a dynamic complex involving other enzymes that manage intracellular energy balance. PGM1 supports glycogen synthesis and breakdown by regulating the availability of glucose-1-phosphate. This dual role makes PGM1 an important protein for maintaining normal metabolic function. Its activity impacts cellular energy levels which is vital for muscle contraction and overall energy distribution in tissues.

Pathways

PGM1 is critically involved in carbohydrate metabolic pathways specifically glycolysis and gluconeogenesis. In glycolysis it participates in converting glucose for energy production. In gluconeogenesis it assists in the generation of glucose from non-carbohydrate substrates. PGM1 interacts closely with other enzymes in these pathways such as glucose-6-phosphate dehydrogenase ensuring efficient energy regulation and metabolic flow within the cell.

PGM1 is associated with glycogen storage disease type XIV which is a condition causing improper glycogen metabolism. Mutations in the PGM1 gene disrupt its enzymatic function leading to symptoms like muscle pain and weakness. Additionally PGM1 defects can impact another protein phosphoglucomutase-3 (PGM3) which shares pathway similarities and potentially affects the synthesis of other phosphorylated sugars related to immune function. These connections highlight the importance of functional PGM1 in maintaining normal physiological and disease-free states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate (PubMed : 15378030, PubMed : 25288802). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable) (PubMed : 25288802). This enzyme participates in both the breakdown and synthesis of glucose (PubMed : 17924679, PubMed : 25288802).

See full target information PGM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGM1 antibody [EPR15240] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (AB250979)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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