Anti-PGM1 antibody [EPR15241] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158)

This data was developed using ab192876, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling PGM1 with ab192876 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stain is DAPI (blue).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158)

This data was developed using ab192876, the same antibody clone in a different buffer formulation.

ab192876 staining PGM1 in A549 (human lung carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/90. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

• IP

Supplier Data

Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158)

This data was developed using ab192876, the same antibody clone in a different buffer formulation.

Western blot analysis of PGM1 immunoprecipitated from Human fetal liver lysate with ab192876 at 1/60 dilution. Lane 1 : Human fetal liver lysate. Lane 2 : PBS instead of Human fetal liver lysate.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (<a href='/en-us/products/primary-antibodies/pgm1-antibody-epr15241-ab192876'>ab192876</a>)

Predicted band size: 61 kDa

false

• WB

Supplier Data

Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158)

This data was developed using ab192876, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PGM1 antibody [EPR15241] (<a href='/en-us/products/primary-antibodies/pgm1-antibody-epr15241-ab192876'>ab192876</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Lane 4:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

• WB

Supplier Data

Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158)

This data was developed using ab192876, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PGM1 antibody [EPR15241] (<a href='/en-us/products/primary-antibodies/pgm1-antibody-epr15241-ab192876'>ab192876</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat kidney tissue lysate at 10 µg

Lane 6:

Rat spleen tissue lysate at 10 µg

Lane 7:

C6 cell lysate at 10 µg

Lane 8:

RAW 264.7 cell lysate at 10 µg

Lane 9:

PC12 cell lysate at 10 µg

Lane 10:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false