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Rabbit Recombinant Monoclonal PGM1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158), expandable thumbnail
  • Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158), expandable thumbnail
  • Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (AB251158), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

5 products for Alternative Version

Target data

Function

Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate (PubMed:15378030, PubMed:25288802). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable) (PubMed:25288802). This enzyme participates in both the breakdown and synthesis of glucose (PubMed:17924679, PubMed:25288802).

Alternative names

Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1

Recommended products

Rabbit Recombinant Monoclonal PGM1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15241
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251158 is the carrier-free version of Anti-PGM1 antibody [EPR15241] ab192876.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Protein PGM1 also known as phosphoglucomutase-1 plays a significant mechanical role in cellular glucose metabolism. It catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate a critical step in the glycolysis and gluconeogenesis pathways. The PGM1 protein has a molecular mass of approximately 61 kDa. Its expression occurs in various tissues with high levels in muscle and liver tissues which are key sites for energy storage and utilization.

Biological function summary

PGM1 contributes significantly to both energy homeostasis and carbohydrate metabolism. It functions as part of a dynamic complex involving other enzymes that manage intracellular energy balance. PGM1 supports glycogen synthesis and breakdown by regulating the availability of glucose-1-phosphate. This dual role makes PGM1 an important protein for maintaining normal metabolic function. Its activity impacts cellular energy levels which is vital for muscle contraction and overall energy distribution in tissues.

Pathways

PGM1 is critically involved in carbohydrate metabolic pathways specifically glycolysis and gluconeogenesis. In glycolysis it participates in converting glucose for energy production. In gluconeogenesis it assists in the generation of glucose from non-carbohydrate substrates. PGM1 interacts closely with other enzymes in these pathways such as glucose-6-phosphate dehydrogenase ensuring efficient energy regulation and metabolic flow within the cell.

Associated diseases and disorders

PGM1 is associated with glycogen storage disease type XIV which is a condition causing improper glycogen metabolism. Mutations in the PGM1 gene disrupt its enzymatic function leading to symptoms like muscle pain and weakness. Additionally PGM1 defects can impact another protein phosphoglucomutase-3 (PGM3) which shares pathway similarities and potentially affects the synthesis of other phosphorylated sugars related to immune function. These connections highlight the importance of functional PGM1 in maintaining normal physiological and disease-free states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158), expandable thumbnail

    Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

    This data was developed using Anti-PGM1 antibody [EPR15241] ab192876, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PGM1 antibody [EPR15241] (Anti-PGM1 antibody [EPR15241] ab192876) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: 293T cell lysate at 20 µg

    Lane 3: Human fetal liver lysate at 20 µg

    Lane 4: Human fetal heart lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

    This data was developed using Anti-PGM1 antibody [EPR15241] ab192876, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling PGM1 with Anti-PGM1 antibody [EPR15241] ab192876 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution. Nuclear counter stain is DAPI (blue).

  • Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158), expandable thumbnail

    Immunoprecipitation - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

    This data was developed using Anti-PGM1 antibody [EPR15241] ab192876, the same antibody clone in a different buffer formulation.Western blot analysis of PGM1 immunoprecipitated from Human fetal liver lysate with Anti-PGM1 antibody [EPR15241] ab192876 at 1/60 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (Anti-PGM1 antibody [EPR15241] ab192876)

    Predicted band size: 61 kDa

  • Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158), expandable thumbnail

    Western blot - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

    This data was developed using Anti-PGM1 antibody [EPR15241] ab192876, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PGM1 antibody [EPR15241] (Anti-PGM1 antibody [EPR15241] ab192876) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Mouse spleen tissue lysate at 10 µg

    Lane 5: Rat kidney tissue lysate at 10 µg

    Lane 6: Rat spleen tissue lysate at 10 µg

    Lane 7: C6 cell lysate at 10 µg

    Lane 8: RAW 264.7 cell lysate at 10 µg

    Lane 9: PC12 cell lysate at 10 µg

    Lane 10: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 61 kDa

  • Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15241] - BSA and Azide free (ab251158)

    This data was developed using Anti-PGM1 antibody [EPR15241] ab192876, the same antibody clone in a different buffer formulation.

    Anti-PGM1 antibody [EPR15241] ab192876 staining PGM1 in A549 (human lung carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/90. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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