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AB249059

Anti-PGM2 antibody [EPR10393(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PGM2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

MSTP006, PGM2, Phosphopentomutase, Glucose phosphomutase 2, Phosphodeoxyribomutase, Phosphoglucomutase-2

1 Images
Western blot - Anti-PGM2 antibody [EPR10393(B)] - BSA and Azide free (AB249059)
  • WB

Unknown

Western blot - Anti-PGM2 antibody [EPR10393(B)] - BSA and Azide free (AB249059)

This data was developed using ab151746, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PGM2 antibody [EPR10393(B)] (<a href='/en-us/products/primary-antibodies/pgm2-antibody-epr10393b-ab151746'>ab151746</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10393(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249059 is the carrier-free version of ab151746.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglucomutase 2 also known as PGM2 is an enzyme involved in carbohydrate metabolism. It catalyzes the reversible conversion of glucose-1-phosphate to glucose-6-phosphate an essential step in glycogenesis and glycogenolysis. PGM2 has a mass of about 65 kDa and is expressed in various tissues including liver muscle and brain. It is often expressed in cells that require significant amounts of energy due to its role in glucose utilization.
Biological function summary

PGM2 functions to maintain cellular energy balance by supporting glycogen storage and breakdown. It operates as an individual enzyme not being part of any multi-enzyme complex but it significantly impacts glucose flux within cells. The enzyme plays a role in both anabolic and catabolic processes highlighting its importance in energy homeostasis. Adequate functioning of PGM2 ensures that cells have access to glucose during fasting or between meals vital for processes like muscle contraction and brain function.

Pathways

PGM2 is actively involved in the glycogen metabolism pathway and interacts with key enzymes like glycogen phosphorylase and glycogen synthase. Its enzymatic activity links it to pathways such as glycolysis where glucose-6-phosphate serves as a substrate. Through its role in glucose conversion PGM2 influences the availability of substrate for additional enzymes in glycolysis and glycogenesis impacting energy production and storage in cells.

Defects in PGM2 have been associated with glycogen storage disease which affects glucose and glycogen metabolism leading to energy production issues. It is also observed in metabolic myopathies where disordered energy metabolism leads to muscle weakness and fatigue. PGM2 interacts with glycogen phosphorylase in these disorders as both play parts in the conversion of glucose-1-phosphate to glucose-6-phosphate implicating their coordination in maintaining metabolic stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of the nucleoside breakdown products ribose-1-phosphate and deoxyribose-1-phosphate to the corresponding 5-phosphopentoses (PubMed : 17804405). Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate but with a lower catalytic efficiency (PubMed : 17804405). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (PubMed : 17804405). In vitro, has also a low glucose 1,6-bisphosphate synthase activity which is most probably not physiologically relevant (PubMed : 17804405, PubMed : 18927083).
See full target information PGM2
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Product promise

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