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AB128094

Anti-PGM3 antibody [OTI1A11]

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(3 Publications)

Mouse Monoclonal PGM3 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, African green monkey, Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGM3.

View Alternative Names

AGM1, PGM3, Phosphoacetylglucosamine mutase, PAGM, Acetylglucosamine phosphomutase, N-acetylglucosamine-phosphate mutase, Phosphoglucomutase-3, PGM 3

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human kidney tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human pancreas tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human liver tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ab128094 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human prostate tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

Paraffin-embedded human prosate carcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • WB

Unknown

Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094)

All lanes:

Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 60 kDa

false

Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094)
  • WB

Unknown

Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094)

All lanes:

Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA at 5 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1A11

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PGM3.

O95394

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

The clone number has been updated from 1A11 to OTI1A11, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglucomutase 3 (PGM3) is a critical enzyme involved in carbohydrate metabolism. It catalyzes the conversion of N-acetylglucosamine-6-phosphate to N-acetylglucosamine-1-phosphate an important step in nucleotide sugar metabolism. PGM3 is also known as AGM1. The molecular weight of PGM3 is approximately 61 kDa. Expression of PGM3 is widespread with significant levels found in liver pancreas and brain tissue indicating its importance in energy metabolism in these organs.
Biological function summary

The role of PGM3 extends beyond simple carbohydrate conversion. PGM3 participates in the synthesis of glycosylation substrates essential for glycan production on proteins and lipids. It functions as a single enzyme not part of a larger complex. Glycosylation is critical for protein folding stability and cell-cell communication and defects in this process can affect numerous cellular activities.

Pathways

PGM3 integrates into the nucleotide sugar metabolism pathway important for glycosylation. It also intersects with the hexosamine biosynthetic pathway. Through these pathways PGM3 directly connects with proteins involved in cellular growth and differentiation such as OGT (O-linked N-acetylglucosamine transferase) which modifies nuclear and cytosolic proteins through O-GlcNAcylation.

PGM3 mutations link to immune system disorders notably immunodeficiency. Genetic variants of PGM3 can result in aberrant glycosylation leading to immune dysfunction and increased susceptibility to infections. Moreover defects in PGM3 are associated with skeletal abnormalities and developmental delays potentially involving interactions with proteins such as N-glycosylated cell surface receptors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways including protein N- and O-glycosylation.
See full target information PGM3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 10: PubMed33670598

2021

Suppression of the HBP Function Increases Pancreatic Cancer Cell Sensitivity to a Pan-RAS Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Ricciardiello,Laura Bergamaschi,Humberto De Vitto,Yang Gang,Taiping Zhang,Roberta Palorini,Ferdinando Chiaradonna

Experimental and therapeutic medicine 13:127-130 PubMed28123480

2017

Correlative study on the JAK-STAT/PSMβ3 signal transduction pathway in asthenozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Junguo Li,Li Zhang,Bing Li

Scientific reports 6:27278 PubMed27255611

2016

Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway.

Applications

WB

Species

Unspecified reactive species

Cédric Chaveroux,Carmen Sarcinelli,Virginie Barbet,Sofiane Belfeki,Audrey Barthelaix,Carole Ferraro-Peyret,Serge Lebecque,Toufic Renno,Alain Bruhat,Pierre Fafournoux,Serge N Manié
View all publications
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