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Mouse Monoclonal PGM3 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, African green monkey, Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGM3.

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Images

Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094), expandable thumbnail
  • Western blot - Anti-PGM3 antibody [OTI1A11] (AB128094), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human PGM3. Database link O95394

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Expected
Mouse
Tested
Expected
Expected
African green monkey
Tested
Expected
Expected
Transfected cell line - Human
Not recommended
Not recommended
Tested
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
African green monkey
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/100
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, African green monkey, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways including protein N- and O-glycosylation.

Alternative names

AGM1, PGM3, Phosphoacetylglucosamine mutase, PAGM, Acetylglucosamine phosphomutase, N-acetylglucosamine-phosphate mutase, Phosphoglucomutase-3, PGM 3

Recommended products

Mouse Monoclonal PGM3 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, African green monkey, Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGM3.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human PGM3. Database link O95394
Clone number
OTI1A11
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from 1A11 to OTI1A11, both clone numbers name the same antibody clone.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Phosphoglucomutase 3 (PGM3) is a critical enzyme involved in carbohydrate metabolism. It catalyzes the conversion of N-acetylglucosamine-6-phosphate to N-acetylglucosamine-1-phosphate an important step in nucleotide sugar metabolism. PGM3 is also known as AGM1. The molecular weight of PGM3 is approximately 61 kDa. Expression of PGM3 is widespread with significant levels found in liver pancreas and brain tissue indicating its importance in energy metabolism in these organs.

Biological function summary

The role of PGM3 extends beyond simple carbohydrate conversion. PGM3 participates in the synthesis of glycosylation substrates essential for glycan production on proteins and lipids. It functions as a single enzyme not part of a larger complex. Glycosylation is critical for protein folding stability and cell-cell communication and defects in this process can affect numerous cellular activities.

Pathways

PGM3 integrates into the nucleotide sugar metabolism pathway important for glycosylation. It also intersects with the hexosamine biosynthetic pathway. Through these pathways PGM3 directly connects with proteins involved in cellular growth and differentiation such as OGT (O-linked N-acetylglucosamine transferase) which modifies nuclear and cytosolic proteins through O-GlcNAcylation.

Associated diseases and disorders

PGM3 mutations link to immune system disorders notably immunodeficiency. Genetic variants of PGM3 can result in aberrant glycosylation leading to immune dysfunction and increased susceptibility to infections. Moreover defects in PGM3 are associated with skeletal abnormalities and developmental delays potentially involving interactions with proteins such as N-glycosylated cell surface receptors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094)

    All lanes: Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA at 5 µg

    Predicted band size: 60 kDa

  • Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094)

    All lanes: Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

    Lane 3: SVT2 cell extract at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) cell lysate at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

    Lane 7: MDCK cell extract at 35 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

    Lane 9: MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human kidney tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human liver tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human pancreas tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human prostate tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Paraffin-embedded human prosate carcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-PGM3 antibody [OTI1A11] (ab128094), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PGM3 antibody [OTI1A11] (ab128094)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ab128094 at 1/100 dilution.

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Product protocols

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