JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242104

Anti-PGP antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal PGP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PGP aa 250 to C-terminus.

View Alternative Names

Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, Phosphoglycolate phosphatase, AUM, PGP

2 Images
Western blot - Anti-PGP antibody (AB242104)
  • WB

Supplier Data

Western blot - Anti-PGP antibody (AB242104)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-PGP antibody (ab242104) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 34 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-PGP antibody (AB242104)
  • IP

Supplier Data

Immunoprecipitation - Anti-PGP antibody (AB242104)

PGP IP in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242104 at 8 μL per reaction. Western blot was performed on the immunoprecipitate using ab242104 at 1/400 dilution.

Lane 1 : ab242104 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-PGP antibody (ab242104)

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PGP aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

A6NDG6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to PGP immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGP also known as P-glycoprotein or Pgp protein functions as an important membrane-associated ATP-dependent efflux transporter. It has a molecular weight of approximately 170 kDa. This protein is widely expressed in cells throughout the body with high levels present in the liver kidney intestine and blood-brain barrier. PGP's mechanical role involves transporting various substrates including drugs metabolic products and xenobiotics across cellular membranes which is important for protecting tissues from toxins.
Biological function summary

PGP plays an important role in the body's defense by contributing to the cellular excretion of potentially harmful substances. It is part of the ATP-binding cassette (ABC) transporters family. This protein helps maintain cellular homeostasis by regulating the intracellular concentration of substrates. PGP products influence pharmacokinetics by affecting drug absorption and excretion which can affect therapy outcomes. The study of PGP alternatives may provide insights into overcoming drug resistance in various conditions.

Pathways

PGP participates in two major biological systems: the metabolic pathway and the detoxification pathway. In these pathways PGP works closely with other proteins such as cytochrome P450 enzymes to metabolize and eliminate substances from cells. Together with multidrug resistance-associated proteins PGP contributes to the intricate network of cellular defense mechanisms. The proper functioning of these pathways is essential for protecting tissues from damage due to toxins and drug accumulation.

PGP's activity is significant in influencing the development of multidrug resistance in cancer treatment and the progression of neurodegenerative diseases like Alzheimer's disease. Altered PGP expression can lead to inefficient drug clearance in cancer making chemotherapy less effective and allowing cancer cells to survive. In Alzheimer's disease overexpression may reduce the clearance of amyloid-beta a protein linked to the condition which leads to its accumulation in the brain. Consequently research into modulating PGP expression and function is critical for improving therapeutic strategies in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed : 26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).
See full target information PGP
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33760140

2021

Inhibition of chaperone‑mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xuan,Shuang Zhao,Xingjun Xiao,Liwei Xiang,Hua-Chuan Zheng

Experimental and therapeutic medicine 18:2731-2738 PubMed31572520

2019

Reduced pim-1 expression increases chemotherapeutic drug sensitivity in human androgen-independent prostate cancer cells by inducing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhang,Yuyan Sun,Peng Wang,Changfu Yang,Shengwei Li
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB262880

Anti-PGP antibody

primary-antibodies

pgp-antibody-ab262880

0

(0 reviews)

Alternative Product
Primary Antibodies

AB261736

Anti-PGP antibody

primary-antibodies

pgp-antibody-ab261736

0

(0 reviews)

Alternative Product
Primary Antibodies

AB234884

Anti-PGP antibody

primary-antibodies

pgp-antibody-ab234884

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com