Rabbit Polyclonal PGP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGP aa 250 to C-terminus.
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- Nature communications 15:36842024Development of Aptamer-DNAzyme based metal-nucleic acid frameworks for gastric cancer therapy.Applications:IHC-PReactive species:HumanJiaqi Yan,Rajendra Bhadane,Meixin Ran,Xiaodong Ma,Yuanqiang Li,Dongdong Zheng,Outi M H Salo-Ahen,Hongbo ZhangPubMed 38693181
- Aging 16:2077-20892023ST-III culture supernatant protects against acute alcohol-induced liver and intestinal injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Xu,Zengqiang Chen,Longteng Xie,Shizhuo Yang,Yuying Li,Junnan Wu,Yuyu Wu,Siyuan Li,Xie Zhang,Yanyan Ma,Yanlong Liu,Aibing Zeng,Zeping XuPubMed 38126998
- Proteomics 22:e22000812022Proteomics reveals MRPL4 as a high-risk factor and a potential diagnostic biomarker for prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQihuan Fu,Ruixia Hong,Hang Zhou,Ying Li,Xiu Liu,Jiaqi Gong,Xiaoyang Wang,Jiajia Chen,Haiying Ran,Liting Wang,Fang Li,Jiangbei YuanPubMed 36059095
- Cancer gene therapy 28:126-1402020MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Li,Tao Wang,Fei-Fan Sun,Jian-Qiong Feng,Jing-Jing Peng,Hua Li,Chao Wang,Dan Wang,Yu Liu,Yu-Di Bai,Mao-Lin Shi,Tao ZhangPubMed 32616906
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PGP aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link A6NDG6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes Recombinant expression validation in WB using target protein overexpression. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).
Alternative names
Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, Phosphoglycolate phosphatase, AUM, PGP
Recommended products
Rabbit Polyclonal PGP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGP aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PGP aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link A6NDG6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGP also known as P-glycoprotein or Pgp protein functions as an important membrane-associated ATP-dependent efflux transporter. It has a molecular weight of approximately 170 kDa. This protein is widely expressed in cells throughout the body with high levels present in the liver kidney intestine and blood-brain barrier. PGP's mechanical role involves transporting various substrates including drugs metabolic products and xenobiotics across cellular membranes which is important for protecting tissues from toxins.
- Biological function summary
PGP plays an important role in the body's defense by contributing to the cellular excretion of potentially harmful substances. It is part of the ATP-binding cassette (ABC) transporters family. This protein helps maintain cellular homeostasis by regulating the intracellular concentration of substrates. PGP products influence pharmacokinetics by affecting drug absorption and excretion which can affect therapy outcomes. The study of PGP alternatives may provide insights into overcoming drug resistance in various conditions.
- Pathways
PGP participates in two major biological systems: the metabolic pathway and the detoxification pathway. In these pathways PGP works closely with other proteins such as cytochrome P450 enzymes to metabolize and eliminate substances from cells. Together with multidrug resistance-associated proteins PGP contributes to the intricate network of cellular defense mechanisms. The proper functioning of these pathways is essential for protecting tissues from damage due to toxins and drug accumulation.
- Associated diseases and disorders
PGP's activity is significant in influencing the development of multidrug resistance in cancer treatment and the progression of neurodegenerative diseases like Alzheimer's disease. Altered PGP expression can lead to inefficient drug clearance in cancer making chemotherapy less effective and allowing cancer cells to survive. In Alzheimer's disease overexpression may reduce the clearance of amyloid-beta a protein linked to the condition which leads to its accumulation in the brain. Consequently research into modulating PGP expression and function is critical for improving therapeutic strategies in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP antibody (ab261736)
Immunohistochemical analysis of human testis tissue labelling PGP with ab261736 shows moderate granular cytoplasmatic positivity in cells in semiferous ducts and Leydig cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP antibody (ab261736)
Immunohistochemical analysis of human cerebral cortex tissue labelling PGP with ab261736 shows strong positivity in neuropil.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP antibody (ab261736)
Immunohistochemical analysis of human skeletal muscle tissue labelling PGP with ab261736 shows weak to moderate cytotoplasmatic positivity in myocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP antibody (ab261736)
Immunohistochemical analysis of human placenta tissue labelling PGP with ab261736 shows moderate granular cytoplasmatic positivity in trophoblastic cells.
Immunocytochemistry/ Immunofluorescence - Anti-PGP antibody (ab261736)
Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling PGP using ab261736 at 4 μg/ml. Fixation/Permeabilization: PFA/Triton X-100.
Western blot - Anti-PGP antibody (ab261736)
All lanes: Western blot - Anti-PGP antibody (ab261736) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T cell lysate
Lane 2: PGP overexpression HEK-293T cell lysate
Predicted band size: 34 kDa
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