Rabbit Polyclonal PGP9.5 antibody. Neural Stem Cell marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 18 publications.
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- Journal of neurology 269:4174-41842022Prognostic value of cutaneous reinnervation with GAP-43 in oxaliplatin-induced neuropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMerve Albayrak,Carolina Figueras,Elia Seguí,Michela Campolo,Eva Gabarrón,Reinaldo Moreno,Joan Maurel,Jordi Casanova-MollaPubMed 35258850
- PLoS computational biology 18:e10099122022Objective quantification of nerves in immunohistochemistry specimens of thyroid cancer utilising deep learning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIndriani P Astono,James S Welsh,Christopher W Rowe,Phillip JoblingPubMed 35226665
- Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 20:881-8902021VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD).Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Semaniakou,Frederic Chappe,Younes Anini,Valerie ChappePubMed 34034984
- Scientific reports 11:73902021Tumor innervation and clinical outcome in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAysha Ferdoushi,Nathan Griffin,Mark Marsland,Xiaoyue Xu,Sam Faulkner,Fangfang Gao,Hui Liu,Simon J King,James W Denham,Dirk F van Helden,Phillip Jobling,Chen Chen Jiang,Hubert HondermarckPubMed 33795769
- Vaccines 9:2021Assessment of Two Novel Live-Attenuated Vaccine Candidates for Herpes Simplex Virus 2 (HSV-2) in Guinea Pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan D Joyce,Anant K Patel,Brandie Murphy,Daniel J J Carr,Edward Gershburg,Andrea S BertkePubMed 33805768
- Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 20:154-1642020Disrupted local innervation results in less VIP expression in CF mice tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Semaniakou,Sarah Brothers,Grayson Gould,Mehrsa Zahiremani,Jamie Paton,Frederic Chappe,Audrey Li,Younes Anini,Roger P Croll,Valerie ChappePubMed 32600901
- FASEB bioAdvances 2:398-4082020Nerve growth factor and its receptor tyrosine kinase TrkA are overexpressed in cervical squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSam Faulkner et. al.PubMed 32676580
- The American journal of pathology 190:1921-19302020Clinicopathological Significance of Nerves in Esophageal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan Griffin et. al.PubMed 32479822
- Scientific reports 10:15392020Innervation of papillary thyroid cancer and its association with extra-thyroidal invasion.
- Science advances 5:eaax47552019Building sensory axons: Delivery and distribution of Na1.7 channels and effects of inflammatory mediators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth J Akin et. al.PubMed 31681845
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Cool at RT for 20 minutes. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).
Alternative names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1
Recommended products
Rabbit Polyclonal PGP9.5 antibody. Neural Stem Cell marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab108986.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
- Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
- Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
- Associated diseases and disorders
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 using ab15503 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)
ICC/IF image of ab15503 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15503 at 1 ug/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)
Lane 1: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab15503) at 1/25 dilution
Lane 1: Western blot - Anti-PGP9.5 antibody, prediluted (Anti-PGP9.5 antibody, prediluted ab15504) at 1/25 dilution
All lanes: Human Brain lysate
Predicted band size: 24 kDa
Observed band size: 26 kDa
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