AB227157

Anti-PGP9.5 antibody - Neuronal Marker

Name: Anti-PGP9.5 antibody - Neuronal Marker (ab227157) | Abcam
Brand: Abcam Limited
SKU: AB227157
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PGP9.5 antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UCHL1.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Paraffin-embedded SEROUS OVCA (human) xenograft tissue stained for PGP9.5 with ab227157 at 1/300 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for PGP9.5 (green) using ab227157 at 1/500 dilution in ICC/IF.

The nuclear counter stain is Fluoroshield with DAPI. Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).

Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Frozen section of E13.5 rat brain tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)

The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin 3 is detected with an anti-beta-Tubulin 3 antibody (red).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Frozen section of adult mouse cerebellum tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)

The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).

Supplier Data

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

12% SDS-PAGE

All lanes:

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/3000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T treated with PGP9.5 shRNA, whole cell extract at 30 µg

Predicted band size: 24 kDa

true

Supplier Data

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

12% SDS PAGE

All lanes:

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/5000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 24 kDa

true

Supplier Data

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

12% SDS-PAGE

All lanes:

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/10000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Predicted band size: 24 kDa

true

Supplier Data

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

12 % SDS-PAGE

All lanes:

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/5000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Predicted band size: 24 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-Fr, IHC-P

applications

Immunogen

Synthetic Peptide within Human UCHL1. The exact immunogen used to generate this antibody is proprietary information.

P09936

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.

Biological function summary

PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.

Pathways

PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.

Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed : 22212137, PubMed : 23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed : 22212137, PubMed : 25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed : 12408865, PubMed : 8639624, PubMed : 9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed : 22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed : 25615526).

See full target information UCHL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology : PubMed32969494

2020

Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance hemodialysis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fulvia Draicchio,Stephan van Vliet,Oana Ancu,Scott A Paluska,Kenneth R Wilund,Monika Mickute,Thozhukat Sathyapalan,Derek Renshaw,Peter Watt,Lykke Sylow,Nicholas A Burd,Richard Mackenzie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGP9.5 antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB227157)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise