Rabbit Polyclonal PGP9.5 antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human UCHL1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UCHL1. The exact immunogen used to generate this antibody is proprietary information. Database link P09936
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Tested | Expected | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/10000 | Notes - |
Species Rat | Dilution info 1/500 - 1/10000 | Notes - |
Species Human | Dilution info 1/500 - 1/10000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).
Alternative names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1
Recommended products
Rabbit Polyclonal PGP9.5 antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human UCHL1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UCHL1. The exact immunogen used to generate this antibody is proprietary information. Database link P09936
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
- Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
- Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
- Associated diseases and disorders
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-PGP9.5 antibody
Paraffin-embedded SEROUS OVCA (human) xenograft tissue stained for PGP9.5 with ab227157 at 1/300 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody (ab227157)
Frozen section of E13.5 rat brain tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)
The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin 3 is detected with an anti-beta-Tubulin 3 antibody (red).
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Western blot staining using rabbit Anti-PGP9.5 antibody
12% SDS-PAGE
All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/3000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: HEK-293T treated with PGP9.5 shRNA, whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody (ab227157)
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for PGP9.5 (green) using ab227157 at 1/500 dilution in ICC/IF.
The nuclear counter stain is Fluoroshield with DAPI. Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Western blot staining of Rat brain tissue extract using rabbit Anti-PGP9.5 antibody
12% SDS-PAGE
All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/10000 dilution
All lanes: Rat brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Immunohistochemistry (Frozen sections) staining using rabbit Anti-PGP9.5 antibody
Frozen section of adult mouse cerebellum tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)
The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Western blot staining using rabbit Anti-PGP9.5 antibody
12% SDS PAGE
All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/5000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 2: H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157)
PGP9.5 Western blot staining of Mouse brain tissue extract using rabbit Anti-PGP9.5 antibody
12 % SDS-PAGE
All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab227157) at 1/5000 dilution
All lanes: Mouse brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com