AB72910

Anti-PGP9.5 antibody - Neuronal Marker

Name: Anti-PGP9.5 antibody - Neuronal Marker (ab72910) | Abcam
Brand: Abcam Limited
SKU: AB72910
Price: 525 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(12 Publications)

Chicken Polyclonal PGP9.5 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UCHL1.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.

Lab

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

Western blot : Anti-PDE5A antibody [EPR24129-95] (ab259945) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab259945 was shown to bind specifically to PDE5A. A band was observed at 95 kDa in wild-type A549 cell lysates with no signal observed at this size in PDE5A knockout cell line. To generate this image, wild-type and PDE5A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PDE5A/PDE5 antibody [EPR24129-95] (<a href='/en-us/products/primary-antibodies/pde5a-pde5-antibody-epr24129-95-ab259945'>ab259945</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human PDE5A knockout A549 cell line (<a href='/en-us/products/cell-lines/human-pde5a-knockout-a549-cell-line-ab301140'>ab301140</a>)

Lane 2:

PDE5A knockout A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 100 kDa

Observed band size: 95 kDa

false

Unknown

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

All lanes:

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910) at 1/2500 dilution

All lanes:

rat hippocampal homogenate

Predicted band size: 24 kDa

Observed band size: 25 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human UCHL1.

P09936

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Storage buffer

Preservative: 0.065% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.

Biological function summary

PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.

Pathways

PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.

Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed : 22212137, PubMed : 23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed : 22212137, PubMed : 25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed : 12408865, PubMed : 8639624, PubMed : 9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed : 22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed : 25615526).

See full target information UCHL1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 5:103057 PubMed38762883

2024

Protocol to culture enteric glial cells from the submucosal and myenteric plexi of neonatal and juvenile pig colons.

Applications

Unspecified application

Species

Unspecified reactive species

Madison L Caldwell,Caleb A Cook,Chloe L Mariant,Melissa Touvron,Jack Odle,Anthony T Blikslager,Amanda L Ziegler,Laurianne Van Landeghem

American journal of physiology. Gastrointestinal and liver physiology 326:G228-G246 PubMed38147796

2023

Enteric glial cell network function is required for epithelial barrier restitution following intestinal ischemic injury in the early postnatal period.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda L Ziegler,Madison L Caldwell,Sara E Craig,Emily A Hellstrom,Anastasia E Sheridan,Melissa S Touvron,Tiffany A Pridgen,Scott T Magness,Jack Odle,Laurianne Van Landeghem,Anthony T Blikslager

Cell reports 42:113071 PubMed37676767

2023

Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kristopher J Schmit,Pierre Garcia,Alessia Sciortino,Velma T E Aho,Beatriz Pardo Rodriguez,Mélanie H Thomas,Jean-Jacques Gérardy,Irati Bastero Acha,Rashi Halder,Camille Cialini,Tony Heurtaux,Irina Ostahi,Susheel B Busi,Léa Grandmougin,Tuesday Lowndes,Yogesh Singh,Eric C Martens,Michel Mittelbronn,Manuel Buttini,Paul Wilmes

Cells 12: PubMed37174648

2023

Glycyrrhizic Acid Prevents Paclitaxel-Induced Neuropathy via Inhibition of OATP-Mediated Neuronal Uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Klein,Jörg Isensee,Martin H J Wiesen,Thomas Imhof,Meike K Wassermann,Carsten Müller,Tim Hucho,Manuel Koch,Helmar C Lehmann

Cell stem cell 29:528-544.e9 PubMed35276096

2022

A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Gadomski,Claire Fielding,Andrés García-García,Claudia Korn,Chrysa Kapeni,Sadaf Ashraf,Javier Villadiego,Raquel Del Toro,Olivia Domingues,Jeremy N Skepper,Tatiana Michel,Jacques Zimmer,Regine Sendtner,Scott Dillon,Kenneth E S Poole,Gill Holdsworth,Michael Sendtner,Juan J Toledo-Aral,Cosimo De Bari,Andrew W McCaskie,Pamela G Robey,Simón Méndez-Ferrer

Brain, behavior, and immunity 76:126-138 PubMed30453020

2018

Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Raymond Wong,Nikolett Lénárt,Laura Hill,Lauren Toms,Graham Coutts,Bernadett Martinecz,Eszter Császár,Gábor Nyiri,Athina Papaemmanouil,Ari Waisman,Werner Müller,Markus Schwaninger,Nancy Rothwell,Sheila Francis,Emmanuel Pinteaux,Adam Denés,Stuart M Allan

Translational neurodegeneration 7:13 PubMed29988485

2018

Age-dependent alpha-synuclein accumulation and aggregation in the colon of a transgenic mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Qian Chen,Caroline Haikal,Wen Li,Ming-Tao Li,Zhan-You Wang,Jia-Yi Li

Scientific reports 8:1838 PubMed29382907

2018

Role of P2X Receptor in Mouse Voiding Function.

Applications

ICC/IF

Species

Unspecified reactive species

Weiqun Yu,Warren G Hill,Simon C Robson,Mark L Zeidel

Frontiers in physiology 8:391 PubMed28642718

2017

Irinotecan-Induced Gastrointestinal Dysfunction Is Associated with Enteric Neuropathy, but Increased Numbers of Cholinergic Myenteric Neurons.

Applications

IHC-P

Species

Mouse

Rachel M McQuade,Vanesa Stojanovska,Elizabeth L Donald,Ahmed A Rahman,Dean G Campelj,Raquel Abalo,Emma Rybalka,Joel C Bornstein,Kulmira Nurgali

British journal of pharmacology 172:4024-37 PubMed25917171

2015

Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2.

Applications

Unspecified application

Species

Unspecified reactive species

Na N Guan,Karl Svennersten,Petra J de Verdier,N Peter Wiklund,Lars E Gustafsson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGP9.5 antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise