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Chicken Polyclonal PGP9.5 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UCHL1.


Images

Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (AB72910), expandable thumbnail
  • Western blot - Anti-PGP9.5 antibody - Neuronal Marker (AB72910), expandable thumbnail

Publications

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

Preservative: 0.065% Sodium azide

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human UCHL1. Database link P09936

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/2500
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/500
Notes

-

Associated Products

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Target data

Function

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).

Alternative names

Recommended products

Chicken Polyclonal PGP9.5 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UCHL1.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human UCHL1. Database link P09936
Purity
IgY fraction
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.

Biological function summary

PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.

Pathways

PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.

Associated diseases and disorders

Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.

Product promise

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3 product images

  • Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910), expandable thumbnail

    Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)

    PGP9.5 Western blot staining of rat hippocampal homogenate using chicken Anti-PGP9.5 antibody

    All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910) at 1/2500 dilution

    All lanes: rat hippocampal homogenate

    Predicted band size: 24 kDa

    Observed band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (ab72910), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)

    PGP9.5 Immunocytochemistry/ Immunofluorescence staining using chicken Anti-PGP9.5 antibody

    ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.

  • Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910), expandable thumbnail

    Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)

    PGP9.5 Western blot staining using chicken Anti-PGP9.5 antibody

    Western blot: Anti-PDE5A antibody [EPR24129-95] (Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945 was shown to bind specifically to PDE5A. A band was observed at 95 kDa in wild-type A549 cell lysates with no signal observed at this size in PDE5A knockout cell line. To generate this image, wild-type and PDE5A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-PDE5A/PDE5 antibody [EPR24129-95] (Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: Western blot - Human PDE5A knockout A549 cell line (Human PDE5A knockout A549 cell line ab301140)

    Lane 2: PDE5A knockout A549 cell lysate at 20 µg

    Lane 3: K562 cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 100 kDa

    Observed band size: 95 kDa

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