Chicken Polyclonal PGP9.5 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UCHL1.
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- Cells 12:2023Glycyrrhizic Acid Prevents Paclitaxel-Induced Neuropathy via Inhibition of OATP-Mediated Neuronal Uptake.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInes Klein,Jörg Isensee,Martin H J Wiesen,Thomas Imhof,Meike K Wassermann,Carsten Müller,Tim Hucho,Manuel Koch,Helmar C LehmannPubMed 37174648
- Cell stem cell 29:528-544.e92022A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephen Gadomski,Claire Fielding,Andrés García-García,Claudia Korn,Chrysa Kapeni,Sadaf Ashraf,Javier Villadiego,Raquel Del Toro,Olivia Domingues,Jeremy N Skepper,Tatiana Michel,Jacques Zimmer,Regine Sendtner,Scott Dillon,Kenneth E S Poole,Gill Holdsworth,Michael Sendtner,Juan J Toledo-Aral,Cosimo De Bari,Andrew W McCaskie,Pamela G Robey,Simón Méndez-FerrerPubMed 35276096
- Brain, behavior, and immunity 76:126-1382018Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaymond Wong et. al.PubMed 30453020
- Translational neurodegeneration 7:132018Age-dependent alpha-synuclein accumulation and aggregation in the colon of a transgenic mouse model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Qian Chen et. al.PubMed 29988485
- Scientific reports 8:18382018Role of P2X Receptor in Mouse Voiding Function.
- Frontiers in physiology 8:3912017Irinotecan-Induced Gastrointestinal Dysfunction Is Associated with Enteric Neuropathy, but Increased Numbers of Cholinergic Myenteric Neurons.
- British journal of pharmacology 172:4024-372015Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa N Guan et. al.PubMed 25917171
- American journal of physiology. Gastrointestinal and liver physiology 304:G1-1120123-D imaging and illustration of mouse intestinal neurovascular complex.Applications:ICC/IFReactive species:MouseYa-Yuan Fu,Shih-Jung Peng,Hsin-Yao Lin,Pankaj J Pasricha,Shiue-Cheng TangPubMed 23086917
- Journal of immunology (Baltimore, Md. : 1950) 189:381-922012Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Allen et. al.PubMed 22661091
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.065% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human UCHL1. Database link P09936
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 | Notes - |
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Target data
Function
Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed:23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed:22212137, PubMed:25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed:12408865, PubMed:8639624, PubMed:9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed:22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed:25615526).
Alternative names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1
Recommended products
Chicken Polyclonal PGP9.5 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UCHL1.
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.065% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human UCHL1. Database link P09936
- Purity
- IgY fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.
- Biological function summary
PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.
- Pathways
PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.
- Associated diseases and disorders
Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)
PGP9.5 Western blot staining of rat hippocampal homogenate using chicken Anti-PGP9.5 antibody
All lanes: Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910) at 1/2500 dilution
All lanes: rat hippocampal homogenate
Predicted band size: 24 kDa
Observed band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)
PGP9.5 Immunocytochemistry/ Immunofluorescence staining using chicken Anti-PGP9.5 antibody
ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.
Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab72910)
PGP9.5 Western blot staining using chicken Anti-PGP9.5 antibody
Western blot: Anti-PDE5A antibody [EPR24129-95] (Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945 was shown to bind specifically to PDE5A. A band was observed at 95 kDa in wild-type A549 cell lysates with no signal observed at this size in PDE5A knockout cell line. To generate this image, wild-type and PDE5A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PDE5A/PDE5 antibody [EPR24129-95] (Anti-PDE5A/PDE5 antibody [EPR24129-95] ab259945) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: Western blot - Human PDE5A knockout A549 cell line (Human PDE5A knockout A549 cell line ab301140)
Lane 2: PDE5A knockout A549 cell lysate at 20 µg
Lane 3: K562 cell lysate at 20 µg
SecondaryLanes 1 - 3: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 95 kDa
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