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AB220735

Anti-PGPEP1 antibody

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(1 Publication)

Rabbit Polyclonal PGPEP1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PGPEP1 aa 1-100.

View Alternative Names

PGPI, PGPEP1, Pyroglutamyl-peptidase 1, 5-oxoprolyl-peptidase, Pyroglutamyl aminopeptidase I, Pyroglutamyl-peptidase I, Pyrrolidone-carboxylate peptidase, PAP-I, PGP-I

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PGPEP1 antibody (AB220735)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGPEP1 antibody (AB220735)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized HepG2 cells labeling PGPEP1 with ab220735 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PGPEP1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NXJ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGPEP1 also known as pyroglutamyl-peptidase I is an enzyme responsible for the removal of pyroglutamyl (pGlu) groups from the N-terminus of peptides and proteins an important step for protein regulation and maturation. The enzyme typically shows broad substrate specificity. PGPEP1 has an approximate mass of 23 kDa and is expressed in various tissues although its expression levels differ depending on the tissue type. Researchers often examine its expression in the liver and kidney where it is significantly active.
Biological function summary

The removal of the pGlu group by PGPEP1 modifies peptide stability and activity impacting processes like metabolism and signal transduction. PGPEP1 operates as a monomer and does not form part of larger protein complexes. By regulating the degradation of pyroglutamylated proteins it plays an important role in maintaining protein homeostasis within cells.

Pathways

PGPEP1 influences pathways such as protein catabolism and peptide hormone processing where the removal of pGlu groups aids in maintaining proteolytic activity and modulating bioactive peptides. Its activity intersects with hormones like thyrotropin-releasing hormone (TRH) which it processes. PGPEP1 also interacts with other enzymes like aminopeptidases involved in sequential processing of peptides after the initial pyroglutamyl removal.

Research indicates a connection between abnormal PGPEP1 activity and metabolic syndromes such as obesity due to its role in hormonal regulation and peptide processing. Studies also link PGPEP1 to cognitive disorders where it affects neuropeptide processing in neural tissues. Its interaction with hormones like TRH highlights a potential relationship with proteins such as thyroxine-binding globulin in managing these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Removes 5-oxoproline from various penultimate amino acid residues except L-proline.
See full target information PGPEP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 70:99-109 PubMed38372107

2024

TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyu Sun,Shan Gong,Suwei Lan,Ying He,Yanhua Sun,Zhengmao Zhang
View all publications
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