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AB224054

Anti-PGRMC1 antibody

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(1 Publication)

Rabbit Polyclonal PGRMC1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PGRMC1 aa 1-150.

View Alternative Names

HPR6.6, PGRMC, PGRMC1, Membrane-associated progesterone receptor component 1, mPR, Dap1, IZA

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Paraffin embedded human liver tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Paraffin embedded human hippocampus tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Western blot - Anti-PGRMC1 antibody (AB224054)
  • WB

Supplier Data

Western blot - Anti-PGRMC1 antibody (AB224054)

All lanes:

Western blot - Anti-PGRMC1 antibody (ab224054) at 1/100 dilution

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HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 22 kDa

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Western blot - Anti-PGRMC1 antibody (AB224054)
  • WB

Unknown

Western blot - Anti-PGRMC1 antibody (AB224054)

All lanes:

Western blot - Anti-PGRMC1 antibody (ab224054) at 0.4 µg/mL

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Human Liver tissue

Predicted band size: 22 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Paraffin embedded human breast cancer tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Negative control : Paraffin embedded human skeletal tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Paraffin embedded human endometrium tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody (AB224054)

Paraffin embedded human kidney tissue stained for PGRMC1 with ab224054 (1/500 dilution) in immunohistochemical analysis.

Western blot - Anti-PGRMC1 antibody (AB224054)
  • WB

Supplier Data

Western blot - Anti-PGRMC1 antibody (AB224054)

All lanes:

Western blot - Anti-PGRMC1 antibody (ab224054) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat cell line) cell lysate

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PGRMC1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O00264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGRMC1 also known as the Progesterone Receptor Membrane Component 1 plays several roles in cellular physiology. This protein weighs around 28 kDa and is expressed across various tissues including liver lung and kidney. Commonly found in the cell membrane PGRMC1 impacts tumor growth and development due to its role in several cellular functions like signal transduction and cholesterol metabolism.
Biological function summary

PGRMC1 interacts with other proteins to influence cell cycle regulation and apoptosis. It is part of protein complexes that include Cytochrome P450 enzymes. These complexes aid in regulating steroidogenesis and drug metabolism. PGRMC1’s involvement in lipid homeostasis and membrane trafficking suggests its significant role in maintaining cell health and function.

Pathways

PGRMC1 integrates into the mevalonate pathway influencing cholesterol synthesis and steroidogenesis. It interacts with proteins such as CYP51A1 and is involved in the Wnt signaling pathway. These interactions affect cellular growth and differentiation linking PGRMC1 to pathway regulation and cellular stress responses.

Researchers associate PGRMC1 with cancer progression and neurological disorders. Its overexpression relates to various cancers augmenting tumorigenic potential. Additionally PGRMC1’s connection to Alzheimer’s disease involves interactions with amyloid precursor proteins. These links highlight PGRMC1’s potential as a therapeutic target in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a progesterone-binding protein complex (PubMed : 28396637). Binds progesterone (PubMed : 25675345). Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity). Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins (PubMed : 27599036). Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization (PubMed : 30443021).
See full target information PGRMC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:112-128 PubMed39974423

2025

Mitochondrial single-stranded DNA binding protein 1 (SSBP1) high expression as a potential biomarker and association with poor prognosis in hepatocellular carcinoma (HCC).

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ru Lin,Jian-Kang Li,Xi Yang,Bao-Ru Dong,Ru-Ai Liu,Xin-Meng Wang,Min Yu,Wei Xiong
View all publications
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