AB194879

Anti-PGRMC1 antibody - C-terminal

Name: Anti-PGRMC1 antibody - C-terminal (ab194879) | Abcam
Brand: Abcam Limited
SKU: AB194879
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PGRMC1 antibody. C-terminal. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PGRMC1 aa 100 to C-terminus.

View Alternative Names

HPR6.6, PGRMC, PGRMC1, Membrane-associated progesterone receptor component 1, mPR, Dap1, IZA

3 Images

Supplier Data

Western blot - Anti-PGRMC1 antibody - C-terminal (AB194879)

Lysates in lanes 1-3 were prepared using RIPA lysis buffer. Lysates in lanes 4-5 were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PGRMC1 antibody - C-terminal (ab194879) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 22 kDa

true

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody - C-terminal (AB194879)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody - C-terminal (AB194879)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling PGRMC1 with ab194879 at a 1/5000 dilution.

Supplier Data

Immunoprecipitation - Anti-PGRMC1 antibody - C-terminal (AB194879)

Detection of PGRMC1 in immunoprecipitates of 293T whole cell lysate prepared using RIPA lysis buffer (1 mg for IP, 20% of IP loaded) using ab194879 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PGRMC1 antibody - C-terminal (ab194879)

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PGRMC1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab194879 was affinity purified using an epitope specific to PGRMC1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGRMC1 also known as the Progesterone Receptor Membrane Component 1 plays several roles in cellular physiology. This protein weighs around 28 kDa and is expressed across various tissues including liver lung and kidney. Commonly found in the cell membrane PGRMC1 impacts tumor growth and development due to its role in several cellular functions like signal transduction and cholesterol metabolism.

Biological function summary

PGRMC1 interacts with other proteins to influence cell cycle regulation and apoptosis. It is part of protein complexes that include Cytochrome P450 enzymes. These complexes aid in regulating steroidogenesis and drug metabolism. PGRMC1’s involvement in lipid homeostasis and membrane trafficking suggests its significant role in maintaining cell health and function.

Pathways

PGRMC1 integrates into the mevalonate pathway influencing cholesterol synthesis and steroidogenesis. It interacts with proteins such as CYP51A1 and is involved in the Wnt signaling pathway. These interactions affect cellular growth and differentiation linking PGRMC1 to pathway regulation and cellular stress responses.

Researchers associate PGRMC1 with cancer progression and neurological disorders. Its overexpression relates to various cancers augmenting tumorigenic potential. Additionally PGRMC1’s connection to Alzheimer’s disease involves interactions with amyloid precursor proteins. These links highlight PGRMC1’s potential as a therapeutic target in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a progesterone-binding protein complex (PubMed : 28396637). Binds progesterone (PubMed : 25675345). Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity). Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins (PubMed : 27599036). Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization (PubMed : 30443021).

See full target information PGRMC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:7199 PubMed33785835

2021

In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc, PLK1 and the AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Ocklenburg,Fabian Neumann,Alexandra Wolf,Julia Vogel,Kirsten Göpelt,Melanie Baumann,Jennifer Baumann,Philip Kranz,Eric Metzen,Ulf Brockmeier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGRMC1 antibody - C-terminal

Western blot - Anti-PGRMC1 antibody - C-terminal (AB194879)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC1 antibody - C-terminal (AB194879)

Immunoprecipitation - Anti-PGRMC1 antibody - C-terminal (AB194879)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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