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AB251875

Anti-PGRMC2 antibody

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(3 Publications)

Rabbit Polyclonal PGRMC2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PGRMC2 aa 1-50.

View Alternative Names

DG6, PMBP, PGRMC2, Membrane-associated progesterone receptor component 2, Progesterone membrane-binding protein, Steroid receptor protein DG6

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PGRMC2 antibody (AB251875)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PGRMC2 antibody (AB251875)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PGRMC2 (green) using ab251875 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC2 antibody (AB251875)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGRMC2 antibody (AB251875)

Paraffin-embedded human liver tissue stained for PGRMC2 using ab251875 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-PGRMC2 antibody (AB251875)
  • WB

Unknown

Western blot - Anti-PGRMC2 antibody (AB251875)

All lanes:

Western blot - Anti-PGRMC2 antibody (ab251875) at 0.4 µg/mL

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Predicted band size: 24 kDa

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Western blot - Anti-PGRMC2 antibody (AB251875)
  • WB

Unknown

Western blot - Anti-PGRMC2 antibody (AB251875)

All lanes:

Western blot - Anti-PGRMC2 antibody (ab251875) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PGRMC2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O15173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGRMC2 also known as Membrane-Associated Progesterone Receptor Component 2 is a protein that plays several roles mechanically in the cell. This protein weighs approximately 22.5 kDa and exists across various tissues including liver adipose tissue and the central nervous system. It exists in cellular membranes where it may facilitate interaction with other cellular components. This specific positioning and distribution allow PGRMC2 to modulate various signaling pathways.
Biological function summary

PGRMC2 participates in multiple cellular processes related to cell survival and metabolism. It often functions as part of larger protein complexes and interacts with other proteins affecting mitochondrial function and steroid signaling. Its biological role includes influencing mitochondrial respiration and energy homeostasis critical processes in numerous cellular functions. Researchers have discovered that PGRMC2 interacts with heme impacting vital cellular and metabolic functions.

Pathways

PGRMC2 is active in steroid hormone signaling and cellular stress response pathways. It interacts with proteins like cytochrome P450 which is significant for the metabolism of various substances and heme oxygenase which regulates the degradation of heme. These interactions highlight PGRMC2's impact on vital pathways involved in maintaining cellular stability and response mechanisms.

Researchers link PGRMC2 to metabolic conditions such as obesity and diabetes. It plays a role in lipid metabolism and energy balance both critical factors in the development of these conditions. Furthermore PGRMC2 interaction with insulin receptor substrates suggests a connection to insulin resistance a common feature of type II diabetes. Understanding PGRMC2's function and its related proteins offers potential insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity). May serve as a universal non-classical progesterone receptor in the uterus (Probable). Intracellular heme chaperone required for delivery of labile, or signaling heme, to the nucleus (By similarity). Plays a role in adipocyte function and systemic glucose homeostasis (PubMed : 28111073). In brown fat, which has a high demand for heme, delivery of labile heme in the nucleus regulates the activity of heme-responsive transcriptional repressors such as NR1D1 and BACH1 (By similarity).
See full target information PGRMC2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40571642

2025

Modulation of Th17/Treg Balance by Total Flavonoids from Semen Cuscutae through PGRMC2 in a Diminished Ovarian Reserve Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhou,Shanshan Xu,Juan Tang,Xiaomei Han,Yu Jiang,Huaying Chen

Heliyon 10:e36570 PubMed39263088

2024

PGRMC2 influences the onset of postmenopausal osteoporosis through disulfidptosis in monocytes: Evidence from experimental validation and Mendelian randomization.

Applications

Unspecified application

Species

Unspecified reactive species

Yaobin Wang,Hefang Xiao,Yi Chen,Xiaoyun Sheng,Zhiwei Feng,Bo Peng,Zhongcheng Liu,Hongwei Zhan,Dejian Xiang,Chengjun Zhang,Yayi Xia,Bin Geng

Heliyon 10:e35303 PubMed39170313

2024

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Luo,Tao Chen,Junyi Deng,Ziyuan Liu,Changlong Bi,Song Lan
View all publications
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