AB80612

Anti-PH3 antibody

Name: Anti-PH3 antibody (ab80612) | Abcam
Brand: Abcam Limited
SKU: AB80612
Price: 535 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(10 Publications)

Rabbit Polyclonal PH3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Drosophila melanogaster samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human PHC3 aa 700-750.

View Alternative Names

EDR3, PH3, PHC3, Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3

2 Images

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Immunoprecipitation - Anti-PH3 antibody (AB80612)

Immunoprecipitation of HeLa whole cell lysate using ab80612 at 3μg/mg lysate (lane 1) or a control IgG (lane 2). Bands were detected by Western blot using ab80612 at 1μg/ml. Chemiluminescence with exposure times of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PH3 antibody (ab80612)

Predicted band size: 106 kDa

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Western blot - Anti-PH3 antibody (AB80612)

All lanes:

Western blot - Anti-PH3 antibody (ab80612) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 106 kDa

Observed band size: 160 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Drosophila melanogaster

Applications

ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human PHC3 aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q8NDX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified using an epitope specific to PH3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PH3 also known as phospho-Histone H3 (Ser10) plays an important role in cellular division processes. This protein is characterized by its molecular weight and is highly expressed during mitosis particularly in the chromatin of dividing cells. Researchers often refer to PH3 in scientific literature by its molecular mass or specific markers associated with it. The expression of PH3 is typically detected in actively dividing tissues making it a useful tool for identifying proliferation rates in various tissues.

Biological function summary

PH3 is involved in chromatin remodeling during mitosis. It is not part of a larger complex but instead operates as a marker for chromosome condensation. When PH3 undergoes phosphorylation it indicates the cell has entered mitosis. This action facilitates chromosome condensation and segregation critical for proper mitotic progression. Studies often note PH3's significant role during the G2/M transition phase.

Pathways

PH3 operates within vital mitotic and cell cycle regulation pathways. It is closely associated with the CDK1/cyclin B complex which plays an essential part in mitotic initiation. During the cell cycle its phosphorylation state serves as an indicator of mitotic activity. Additionally other proteins such as Aurora B kinase influence PH3's activity further integrating it into the regulatory network controlling cell division.

Increased PH3 expression correlates with various cancers such as breast cancer and melanoma. This overexpression often results from dysregulation of normal cell cycle mechanisms where PH3's activity becomes abnormally high. Oncological studies link PH3 with other proteins such as p53 where mutations can influence the transition from normal cell activity to oncogenesis impacting disease progression and prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

See full target information PHC3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:1524-1551 PubMed39816679

2025

Cardiomyocyte S1PR1 promotes cardiac regeneration via AKT/mTORC1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxiang Liu,Jinnan Yue,Caixia Zhou,Yunhao Duan,Xiaoli Chen,Jie Liu,Shougang Zhuang,Yu Luo,Jinjin Wu,Yuzhen Zhang,Lin Zhang

Cell proliferation 58:e13765 PubMed39407407

2024

Gdf11 regulates left-right asymmetry development through TGF-β signal.

Applications

Unspecified application

Species

Unspecified reactive species

Wantao Yao,Zhaohui Wei,Xinning Tian,Jin Tan,Jingwen Liu

iScience 27:109967 PubMed38827400

2024

Defining cis-regulatory elements and transcription factors that control human cortical interneuron development.

Applications

Unspecified application

Species

Unspecified reactive species

Gareth Chapman,Julianna Determan,Haley Jetter,Komal Kaushik,Ramachandran Prakasam,Kristen L Kroll

Cells 13: PubMed38667283

2024

Reversal of Postnatal Brain Astrocytes and Ependymal Cells towards a Progenitor Phenotype in Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Kakogiannis,Michaela Kourla,Dimitrios Dimitrakopoulos,Ilias Kazanis

iScience 26:106396 PubMed37013185

2023

JLP/Foxk1/N-cadherin axis fosters a partial epithelial-mesenchymal transition state in epithelial tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maoqing Tian,Lu Zhang,Meng Zhang,Liwen Qiao,Bingqing Xu,Chen Li,Shan Liu,Yuan Song,Zhongping Wei,Yujuan Wang,Huiming Wang

Development (Cambridge, England) 148: PubMed33982759

2021

A multi-gene knockdown approach reveals a new role for Pax6 in controlling organ number in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Alison J Ordway,Gary M Teeters,Bonnie M Weasner,Brandon P Weasner,Robert Policastro,Justin P Kumar

Nature communications 11:4038 PubMed32788587

2020

Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Zhen Li,David Sievert,Desirée E C Smith,Marisa I Mendes,Dillon Y Chen,Valentina Stanley,Shereen Ghosh,Yulu Wang,Majdi Kara,Ayca Dilruba Aslanger,Rasim O Rosti,Henry Houlden,Gajja S Salomons,Joseph G Gleeson

Disease models & mechanisms 13: PubMed32152180

2020

Zika virus non-structural protein NS4A restricts eye growth in through regulation of JAK/STAT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sneh Harsh,Yulong Fu,Eric Kenney,Zhe Han,Ioannis Eleftherianos

The Journal of endocrinology 244:487-499 PubMed31910154

2020

Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyuan Zheng,Hesheng Xiao,Hongjuan Shi,Tingru Wang,Lina Sun,Wenjing Tao,Thomas D Kocher,Minghui Li,Deshou Wang

Developmental biology 360:391-402 PubMed22019301

2011

A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity.

Applications

ICC/IF

Species

Drosophila melanogaster

Rhea R Datta,Brandon P Weasner,Justin P Kumar

View all publications

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Product promise

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Anti-PH3 antibody

Immunoprecipitation - Anti-PH3 antibody (AB80612)

Western blot - Anti-PH3 antibody (AB80612)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise