Rabbit Polyclonal PHAP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANP32A.
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- Journal of Cancer 14:2759-27702023ANP32A Knockdown Attenuates the Malignant Biological Behavior of Colorectal Cancer Cells by Suppressing Epithelial-mesenchymal Transition and ERK Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Li,Xumei Li,Guoxiang Liu,Luwei Zhou,Yisa Liu,Tong Dou,Xu Chen,Juan WangPubMed 37781083
- Investigative ophthalmology & visual science 53:1936-452012Creatine transporter immunolocalization in aged human and detached retinas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClairton F de Souza et. al.PubMed 22410560
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANP32A. The exact immunogen used to generate this antibody is proprietary information. Database link P39687
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed:10400610, PubMed:11360199, PubMed:16341127, PubMed:18439902). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed:18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed:11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed:16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed:18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed:15642345, PubMed:17557114). (Microbial infection) Plays an essential role in influenza A, B and C viral genome replication (PubMed:30666459, PubMed:32694517, PubMed:33045004, PubMed:33208942). Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region (PubMed:33208942). Also plays an essential role in foamy virus mRNA export from the nucleus (PubMed:21159877).
Alternative names
C15orf1, LANP, MAPM, PHAP1, ANP32A, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, pp32, PHAPI
Recommended products
Rabbit Polyclonal PHAP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANP32A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANP32A. The exact immunogen used to generate this antibody is proprietary information. Database link P39687
- Purification technique
- Ion exchange chromatography DEAE-C
- Specificity
This antibody does not cross-react with PHAP12a or PHAPIII.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHAP1 also known as putative HLA-associated protein 1 or ANP32A is a multifunctional phosphoprotein with a molecular weight of approximately 32 kDa. It exists within the nucleus and cytoplasm indicating its role in various cellular processes. PHAP1 expression is found across diverse tissues showing its widespread functional requirements in different biological contexts.
- Biological function summary
PHAP1 plays an important role in regulating apoptosis and cell proliferation. It interacts with components of the INHAT complex which inhibits histone acetylation impacting chromatin remodeling and gene expression. By doing so it influences many cellular events such as cell cycle progression and differentiation. PHAP1's involvement in regulating cell death makes it a target for studying mechanisms underlying cell survival.
- Pathways
PHAP1 is associated with apoptosis and cell signaling processes. It is intricately linked with the p38 MAPK signaling pathway important in stress response and inflammation. In these contexts PHAP1 interacts with proteins such as caspases and protein phosphatase 2A which are important for cellular responses to external and internal stimuli.
- Associated diseases and disorders
PHAP1 shows connections with cancer and neurodegenerative diseases. Altered expression levels of PHAP1 have been observed in certain types of cancers possibly affecting tumor growth and treatment responses. In neurodegeneration relations have been reported with tau proteins suggesting PHAP1's role in tauopathies potentially influencing disease progression and providing insights into therapeutic targets.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-PHAP1 antibody (ab5992)
False colour image of Western blot: Anti-PHAP1 antibody staining at 0.5 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab5992 was shown to bind specifically to PHAP1. A band was observed at 33 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in ANP32A knockout cell line Human ANP32A (PHAP1) knockout HEK-293T cell line ab266148 (knockout cell lysate Human ANP32A (PHAP1) knockout HEK-293T cell lysate ab258303). To generate this image, wild-type and ANP32A knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween� 20 (TBS-T) before incubation with primary antibodies overnight at 4 �C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye� 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye� 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Lanes 1 - 4: Western blot - Anti-PHAP1 antibody (Anti-PHAP1 antibody ab5991) at 0.5 µg/mL
Lanes 1 - 4: Western blot - Anti-PHAP1 antibody (ab5992) at 0.5 µg/mL
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: ANP32A knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human ANP32A (PHAP1) knockout HEK-293T cell line (Human ANP32A (PHAP1) knockout HEK-293T cell line ab266148)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: THP-1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 33 kDa
Western blot - Anti-PHAP1 antibody (ab5992)
All lanes: Western blot - Anti-PHAP1 antibody (ab5992) at 2 µg/mL
Lane 1: Daudi cell lysates at 15 µg
Lane 2: HeLa cell lysates at 15 µg
Lane 3: HepG2 cell lysates at 15 µg
Lane 4: K562 cell lysates at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Western blot - Anti-PHAP1 antibody (ab5992)
Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 μg/ml (lane A) and 4 μg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
All lanes: Western blot - Anti-PHAP1 antibody (ab5992)
Predicted band size: 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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