Anti-PHAP1 antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody - C-terminal (AB189110)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labelling PHAP1 with ab189110 at 10 μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody - C-terminal (AB189110)

Immunofluorescent analysis of Mouse small intestine cells labelling PHAP1 with ab189110 at 20 μg/ml.

• WB

Lab

Western blot - Anti-PHAP1 antibody - C-terminal (AB189110)

False colour image of Western blot : Anti-PHAP1 antibody - C-terminal staining at 0.5 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab189110 was shown to bind specifically to PHAP1. A band was observed at 33 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in ANP32A knockout cell line ab266148 (knockout cell lysate ab258303). To generate this image, wild-type and ANP32A knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-PHAP1 antibody - C-terminal (ab189110) at 0.5 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

ANP32A knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human ANP32A (PHAP1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-anp32a-phap1-knockout-hek-293t-cell-line-ab266148'>ab266148</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 28 kDa

Observed band size: 33 kDa

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• WB

Supplier Data

Western blot - Anti-PHAP1 antibody - C-terminal (AB189110)

All lanes:

Western blot - Anti-PHAP1 antibody - C-terminal (ab189110) at 1 µg/mL

Lane 1:

Raji cell lysate

Lane 2:

Mouse testis tissue lysate

Lane 3:

Rat testis tissue lysate

Predicted band size: 28 kDa

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