Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)

Rabbit Recombinant Monoclonal PHC2 antibody. Suitable for ChIP-seq, WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (AB314436), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIP-seq	WB	ICC/IF	IP	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Not recommended	Tested
Mouse	Expected	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Tested	Expected	Expected	Expected	Tested
Recombinant fragment - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information PHC2

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Alternative names

EDR2, PH2, PHC2, Polyhomeotic-like protein 2, hPH2, Early development regulatory protein 2

Recommended products

Rabbit Recombinant Monoclonal PHC2 antibody. Suitable for ChIP-seq, WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR27147-176
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for FC-intra human.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PHC2 also known as Polyhomeotic Homolog 2 is a protein with a molecular mass around 100 kDa. It localizes mainly in the nucleus and expresses widely across different tissue types. The protein is a part of the Polycomb group (PcG) proteins which play key roles in chromatin remodeling and gene silencing. PHC2 contributes to forming stable gene repression essential for regulating development and maintaining cellular identity.

Biological function summary

PHC2 functions as a part of the Polycomb repressive complex 1 (PRC1). This complex is involved in modifying the structure of chromatin which helps maintain the repression of gene transcription. Through interaction with other PRC1 components PHC2 helps establish a compact chromatin state promoting histone modifications that lead to transcriptional silencing. This activity is fundamental for cellular processes like embryonic development and stem cell maintenance.

Pathways

PHC2 plays a role in the regulation of the Wnt signaling and Notch signaling pathways. These pathways are integral to controlling cell proliferation differentiation and apoptosis. Within these pathways PHC2 interacts with other proteins like BMI1 and RING1B which are also part of the PRC1 complex. These interactions ensure the precise control over gene expression needed for cellular homeostasis and proper developmental progression.

Associated diseases and disorders

Aberrant PHC2 function is implicated in a range of conditions particularly cancer and neurodevelopmental disorders. Dysregulation of PHC2 can lead to misexpression of genes that regulate cell cycle and apoptosis contributing to oncogenesis. For instance PHC2 has connections with BMI1 in cancer pathways influencing tumor progression and resistance to apoptosis. Moreover mutations or irregular expression of PHC2 have been linked with neurodevelopmental disorders highlighting its importance in nervous system development and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

18 product images

Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
PHC2 Western blot staining using rabbit Anti-PHC2 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 16024804).
The identity of the bands below 31 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: Lane1: 180 seconds, lane2-7: 37 seconds
All lanes: Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/1000 dilution
Lane 1: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2 - 3: Human testis tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Rat testis tissue lysate at 20 µg
Lane 7: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 31 kDa, 36 kDa, 40 kDa
Flow Cytometry (Intracellular) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling PHC2 with ab314436 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
ChIP-sequencing - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab314436 [EPR27147-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab314436 [EPR27147-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab314436 [EPR27147-176]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 16024804).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: SW480 transfected with siRNA specifically targeti PHC2 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 31 kDa, 36 kDa, 40 kDa
Exposure time: 80s
Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: Raji (PMID:9121482)
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 16024804).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 31 kDa, 36 kDa, 40 kDa
Exposure time: 92s
Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not react with human PHC1 and PHC3.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
All lanes: Western blot - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/1000 dilution
Lane 1: His-tagged human PHC2 recombinant protein at 10 ng
Lane 2: His-tagged human PHC1 recombinant protein at 10 ng
Lane 3: His-tagged human PHC3 recombinant protein at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 14 kDa
Exposure time: 48s
Immunoprecipitation - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
PHC2 was immunoprecipitated from 0.35 mg SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab314436 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314436 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab314436 IP in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314436 in SW480 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/30 dilution
All lanes: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 58s
Immunoprecipitation - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
PHC2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab314436 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314436 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: ab314436 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314436 NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 76s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human kidney.The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human cerebrum. The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse kidney. The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat kidney. The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling PHC2 with ab314436 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The section was incubated with ab314436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling PHC2 with ab314436 at 1/50 (10.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in SW480 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PHC2 antibody [EPR27147-176] - ChIP Grade (ab314436)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling PHC2 with ab314436 at 1/50 (10.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.