AB80042

Anti-PHD finger protein 1/PHF1 antibody

Name: Anti-PHD finger protein 1/PHF1 antibody (ab80042) | Abcam
Brand: Abcam Limited
SKU: AB80042
Price: 475 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal PHF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PCL1, PHF1, PHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 1/PHF1 antibody (AB80042)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 1/PHF1 antibody (AB80042)

ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.

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Western blot - Anti-PHD finger protein 1/PHF1 antibody (AB80042)

This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1.

All lanes:

Western blot - Anti-PHD finger protein 1/PHF1 antibody (ab80042) at 1 µg/mL

All lanes:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 62 kDa

Observed band size: 50 kDa,62 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to PHD finger protein 1. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab184951.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PHD finger protein 1 also known as PHF1 is a protein involved in chromatin remodeling. It weighs approximately 60 kDa. You may find PHF1 expressed in various human tissues including the brain heart and skeletal muscle which suggests its versatile roles across different cell types. This protein contains a plant homeodomain (PHD) finger motif which functions mechanically in binding to specific histone modifications notably those associated with methylated lysine residues.

Biological function summary

PHD finger protein 1 plays a role in regulating gene expression by modifying the chromatin structure. It acts as part of the PRC2 complex which is pivotal for depositing methyl marks on histone H3 at lysine 27 (H3K27me3). This modification results in transcriptional repression of target genes. Additionally PHF1 interacts with other chromatin regulators thereby contributing to the finely-tuned control of genetic programs essential for cellular differentiation and development.

Pathways

PHD finger protein 1 is involved in the Polycomb group (PcG) pathways along with the Wnt signaling pathway. PHF1 cooperates with other proteins like EZH2 which is an important enzymatic component of the PRC2 complex to mediate its repressive function on chromatin. This interaction and pathway association highlight its involvement in maintaining stem cell pluripotency and lineage specification during development.

PHD finger protein 1 associates with cancers notably endometrial cancer and sarcomas. Alterations in PHF1 expression or function influence the disease progression partly through its interaction with EZH2 leading to abnormal silencing of tumor suppressor genes. Investigations of PHF1 reveal its potential as a biomarker and therapeutic target connecting its modulation to disease outcomes and treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex : it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed : 18285464, PubMed : 23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed : 23142980). Regulates p53/TP53 stability and prolonges its turnover : may act by specifically binding to a methylated from of p53/TP53.

See full target information PHF1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32869745

2020

Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Dong,Reiko Nakagawa,Kyohei Oyama,Yusuke Yamamoto,Weilian Zhang,Aiping Dong,Yanjun Li,Yuriko Yoshimura,Hiroyuki Kamiya,Jun-Ichi Nakayama,Jun Ueda,Jinrong Min

CNS neuroscience & therapeutics 26:228-239 PubMed31364823

2019

Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Yun Liu,Yan Chen,Qian Li,Charles Marshall,Ting Wu,Gang Hu,Ming Xiao

Cell cycle (Georgetown, Tex.) 17:2447-2459 PubMed30382790

2018

PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qu,Yang Wang,Jie Qiao

Brain pathology (Zurich, Switzerland) 29:176-192 PubMed30192999

2018

Deep cervical lymph node ligation aggravates AD-like pathology of APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Linmei Wang,Yanli Zhang,Ying Zhao,Charles Marshall,Ting Wu,Ming Xiao

Nucleic acids research 46:6608-6626 PubMed29846670

2018

PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqiong Liu,Jie Gao,Yang Yang,Rongfang Qiu,Yu Zheng,Wei Huang,Yi Zeng,Yongqiang Hou,Shuang Wang,Shuai Leng,Dandan Feng,Wenqian Yu,Gancheng Sun,Hang Shi,Xu Teng,Yan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHD finger protein 1/PHF1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 1/PHF1 antibody (AB80042)

Western blot - Anti-PHD finger protein 1/PHF1 antibody (AB80042)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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