Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

Intracellular flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184951).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

This data was developed using the same antibody clone in a different buffer formulation (ab184951).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells and 5 µg of ab184951 [EPR14222]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

This data was developed using the same antibody clone in a different buffer formulation (ab184951).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells and 5 µg of ab184951 [EPR14222]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (AB232601)

This data was developed using the same antibody clone in a different buffer formulation (ab184951).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells and 5 µg of ab184951 [EPR14222]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.