Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (AB170929)

Immunofluorescence analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab170929 at 1/100 dilution.

• WB

Lab

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (AB170929)

Lanes 1-4 : Merged signal (red and green). Green - ab170929 observed at 43 kDa. Red - loading control ab7291 observed at 50 kDa.

ab170929 Recombinant Anti-PHF6 antibody [EPR11996(B)] was shown to specifically react with PHF6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264902 (knockout cell lysate ab258110) was used. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. ab170929 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PHF6 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PHF6 (PHD finger protein 6) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-phf6-phd-finger-protein-6-knockout-hela-cell-line-ab264902'>ab264902</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

PHF6 knockout HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 43 kDa

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• WB

Lab

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (AB170929)

Lanes 1 - 4 : Merged signal (red and green). Green - ab170929 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab170929 was shown to recognize PHD finger protein 6/PHF6 in wild-type HEK 293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab170929 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/5000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

PHF6 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PHF6 (PHD finger protein 6) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-phf6-phd-finger-protein-6-knockout-hek-293-cell-line-ab261722'>ab261722</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 41 kDa

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• WB

Supplier Data

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (AB170929)

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/1000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

293T cell lysates at 10 µg

Predicted band size: 41 kDa

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