AB173304

Anti-PHD finger protein 6/PHF6 antibody [EPR11997]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal PHD finger protein 6/PHF6 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CENP-31, KIAA1823, PHF6, PHD finger protein 6, PHD-like zinc finger protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.

Supplier Data

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 41 kDa

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Lab

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

Lanes 1 - 4 : Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

PHF6 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 41 kDa

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Carrier free

Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11997

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHD finger protein 6 also known as PHF6 is a protein with a molecular mass of approximately 43 kDa. It consists of two PHD domains which are zinc finger-like motifs playing roles in chromatin remodeling. PHF6 expresses widely in various tissues with noticeable levels in the brain and hematopoietic cells indicating its significance in these areas. Alternate names for PHF6 include ZNF312 and BORJ although PHF6 is the most commonly referred designation.

Biological function summary

PHF6 involves a range of cellular processes including regulation of transcription and chromatin structure modification. PHF6 can bind to certain DNA sequences and proteins to influence gene expression. It interacts with chromatin remodeling complexes indicating a role in maintaining chromatin accessibility for transcription factors. The protein's PHD domains contribute to its ability to engage in these interactions impacting the transcriptional activity of target genes.

Pathways

PHF6 interacts with transcriptional and chromatin remodeling pathways. It plays a role in the interplay between chromatin and transcriptional machinery which is critical for regulating gene expression. PHF6 associates with proteins such as nucleophosmin and other chromatin regulators influencing pathways like the Notch signaling pathway which is vital for cellular differentiation and development. These interactions highlight its involvement in genetic regulation within developmental pathways.

Mutations in PHF6 link to Börjeson-Forssman-Lehmann syndrome (BFLS) a rare genetic disorder affecting neurological development. Additionally PHF6 mutations recurrently appear in T-cell acute lymphoblastic leukemia (T-ALL) highlighting a role in hematological malignancies. In the context of T-ALL PHF6 interacts with proteins like Notch1 which drives leukemic proliferation when dysregulated. These associations make PHF6 a significant focus for understanding and potentially targeting specific genetic disorders and cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.

See full target information PHF6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:108051 PubMed37942009

2023

Long non-coding RNA regulates cardiomyocyte differentiation through H2A.Z-mediated LHX1 transcriptional activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yu,Benzhi Cai,Yong Zhang,Juan Xu,Dongping Liu,Xiyang Zhang,Zhenbo Han,Yingying Ma,Lei Jiao,Manyu Gong,Xuewen Yang,Yanying Wang,Haodong Li,Lihua Sun,Yu Bian,Fan Yang,Lina Xuan,Haodi Wu,Baofeng Yang,Ying Zhang

Journal of translational medicine 21:220 PubMed36967443

2023

PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Gao,Wensheng Zhang,Jingjing Ma,Xiaojian Ni

Journal of cellular and molecular medicine 27:609-621 PubMed36756714

2023

PHF6 promotes the progression of endometrial carcinoma by increasing cancer cells growth and decreasing T-cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Wang,Aizhong Fang,Yichen Peng,Jianyu Yu,Chunna Yu,Jinxuan Xie,Yi Zheng,Lairong Song,Parker Li,Jia Li,Xun Kang,Yi Lin,Wenbin Li

Molecular medicine reports 23: PubMed33537816

2021

Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Cai,Wenquan Wu,Longhua Guo,Yongwu Xia,Xiaoxin Jiang,Limei Zhang,Feiding Peng,Pinghong Ming

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHD finger protein 6/PHF6 antibody [EPR11997]

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (AB173304)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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