AB226890

Anti-PHD2 / prolyl hydroxylase antibody - C-terminal

Name: Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890) | Abcam
Brand: Abcam Limited
SKU: AB226890
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal PHD2 / prolyl hydroxylase antibody. C-terminal. Suitable for WB, ICC/IF, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EGLN1.

View Alternative Names

C1orf12, PNAS-118, PNAS-137, EGLN1, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, SM-20, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, PHD2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

U-87 MG cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

IV9 rat E18 primary cortical neuron cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.

Immunohistochemistry (Frozen sections) - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

Frozen-sectioned adult mouse cerebellum tissue stained for PHD2 / prolyl hydroxylase using ab226890 at 1/250 dilution in immunohistochemical analysis.

Red : NF-H, stained by NF-H antibody diluted at 1/500.
Blue : DAPI.

Supplier Data

Western blot - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890) at 1/2000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 200 µM cobalt (II) chloride for 24 hours whole cell extract at 30 µg

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EGLN1. The exact immunogen used to generate this antibody is proprietary information.

Q9GZT9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PHD2 also known as prolyl hydroxylase 3 EGLN1 or 366G is a member of the prolyl hydroxylase family with a molecular mass of approximately 46 kDa. PHD2 is expressed in a variety of tissues particularly those in the human body's oxygen-sensing cellular machinery. This protein functions mechanically by adding hydroxyl groups to proline residues on its target proteins an enzymatic activity essential for its role in cellular oxygen sensing.

Biological function summary

This hydroxylation process influences the stability of hypoxia-inducible factor (HIF) by marking it for degradation under normal oxygen levels. PHD2 is a monomeric enzyme but its hydroxylase activity does not require association with other proteins to function. By regulating HIF PHD2 plays a critical role in cellular responses to oxygen availability controlling genes involved in processes such as angiogenesis metabolism and cell survival.

Pathways

PHD2 participates in the HIF signaling pathway and oxygen homeostasis pathways. The regulation of HIF by PHD2 occurs alongside PHD1 and PHD3 which have similar hydroxylase activities. Importantly PHD2 protects cells from hypoxia-related stress by ensuring the degradation of HIF-α subunits under normoxic conditions thereby maintaining cellular homeostasis and proper metabolic function.

PHD2 has connections with conditions such as cancer and chronic kidney disease. Its role in cancer is linked to its regulation of HIF where dysregulation can lead to abnormal cell growth and angiogenesis. In chronic kidney disease the insights into PHD2's function offer potential therapeutic targets particularly since HIF stabilization can ameliorate anemia frequently associated with this condition. Interactions between PHD2 and other proteins in these disease pathways make it a significant focal point for research aimed at developing new treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.

See full target information EGLN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:479 PubMed39134988

2024

Oral administration of Robinia pseudoacacia L. flower exosome-like nanoparticles attenuates gastric and small intestinal mucosal ferroptosis caused by hypoxia through inhibiting HIF-1α- and HIF-2α-mediated lipid peroxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Wang,Heng Zhang,Xingchen Liao,Jun Li,Jie Zeng,Yilin Wang,Mingjie Zhang,Xianzong Ma,Xin Wang,Fangli Ren,Yinyin Wang,Meng Li,Junfeng Xu,Peng Jin,Jianqiu Sheng

Cellular and molecular life sciences : CMLS 81:215 PubMed38739166

2024

Prenatal treatment with preimplantation factor improves early postnatal neurogenesis and cognitive impairments in a mouse model of Down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Manon Moreau,Rodolphe Dard,Amélia Madani,Janany Kandiah,Nadim Kassis,Jessica Ziga,Héloïse Castiglione,Solenn Day,Thomas Bourgeois,Boris Matrot,François Vialard,Nathalie Janel

BMC pulmonary medicine 23:386 PubMed37828459

2023

Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xi,Yan Ma,Dongmei Liu,Rong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PHD2 / prolyl hydroxylase antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

Immunocytochemistry/ Immunofluorescence - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

Immunohistochemistry (Frozen sections) - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

Western blot - Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (AB226890)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise