Rabbit Polyclonal PHF10 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHF10 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
BAF45A, BAF45A, PHF10, PHD finger protein 10, BRG1-associated factor 45a, XAP135, BAF45a
Rabbit Polyclonal PHF10 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PHF10 aa 200 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
PHF10 also known as PHD finger protein 10 plays a mechanistic role in chromatin remodeling and gene expression. It exhibits a molecular weight of approximately 50 kDa. Researchers have identified PHF10 as a component of the BAF (BRG1-associated factor) chromatin remodeling complex. It is widely expressed in various tissues including the brain where it contributes to neural development and function. Its expression can have tissue-specific variations that reflect its diverse roles in different cellular contexts.
PHD finger protein 10 participates in the regulation of gene transcription and chromatin architecture. It functions as part of the BAF complex which orchestrates the dynamic remodeling of chromatin to either activate or repress specific genes. This action facilitates cellular processes like differentiation proliferation and response to environmental signals. PHF10 through its interaction with other BAF complex components influences the accessibility of DNA to transcription factors and other regulatory proteins thereby modulating gene expression patterns in cells.
PHF10 integrates into the Wnt signaling pathway which is important for cell proliferation and differentiation as well as the Hedgehog signaling pathway important for developmental processes. Within these pathways PHF10 collaborates closely with proteins such as SMARCA4 a core ATPase subunit of the BAF complex. These pathways play fundamental roles in ensuring proper development and maintaining cellular homeostasis where PHF10 activity impacts both normal cellular function and response to environmental changes.
Dysregulation of PHF10 function associates with neurodevelopmental disorders and certain forms of cancer. Altered PHF10 expression or mutations can disrupt normal chromatin remodeling activities leading to improper gene expression profiles. In the context of neurodevelopmental disorders PHF10 misfunction can impact genes critical for nervous system development. In some cancers abnormal interactions between PHF10 and proteins like SMARCC1 another BAF complex member can result in the derangement of gene regulatory networks therefore promoting oncogenic processes.
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Lanes 1 - 4: Merged signal (red and green). Green - ab154637 observed at 60 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154637 was shown to react with PHF10 in wild-type HEK-293 cells in Western blot with loss of signal observed in PHF10 knockout sample. Wild-type HEK-293 and PHF10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154637 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PHF10 antibody (ab154637) at 1/500 dilution
Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: PHF10 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: HeLa (Nuclear prepped) cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 54 kDa, 60 kDa
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PHF10 with ab154637 at a 1/500 dilution. Alpha-tubulin filaments were labeled (red) at 1/2000.
Immunohistochemical analysis of paraffin embedded Hela xenograft labeling PHF10 with ab154637 at a 1/500 dilution.
10% SDS PAGE
All lanes: Western blot - Anti-PHF10 antibody (ab154637) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 56 kDa
10 % SDS-PAGE
All lanes: Western blot - Anti-PHF10 antibody (ab154637) at 1/1000 dilution
All lanes: mouse colon lysate/ extract at 50 µg
Predicted band size: 25 kDa, 29 kDa, 44 kDa, 56 kDa
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