JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259946

Anti-PHF10 antibody [EPR23933-210]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PHF10 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

BAF45A, PHF10, PHD finger protein 10, BRG1-associated factor 45a, XAP135, BAF45a

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody [EPR23933-210] (AB259946)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody [EPR23933-210] (AB259946)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling PHF10 with ab259946 at 1/50 (9.86 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse testis. The section was incubated with ab259946 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody [EPR23933-210] (AB259946)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody [EPR23933-210] (AB259946)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling PHF10 with ab259946 at 1/50 (9.86 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat testis. The section was incubated with ab259946 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunoprecipitation - Anti-PHF10 antibody [EPR23933-210] (AB259946)
  • IP

Supplier Data

Immunoprecipitation - Anti-PHF10 antibody [EPR23933-210] (AB259946)

PHF10 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab259946 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259946 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab259946 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259946 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-PHF10 antibody [EPR23933-210] (ab259946)

Predicted band size: 56 kDa

Observed band size: 50-70 kDa

false

Western blot - Anti-PHF10 antibody [EPR23933-210] (AB259946)
  • WB

Lab

Western blot - Anti-PHF10 antibody [EPR23933-210] (AB259946)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 24763304, PMID : 31012017).

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Exposure time : Lanes 1-3 : 59 seconds; Lane 4 : 26 seconds.

All lanes:

Western blot - Anti-PHF10 antibody [EPR23933-210] (ab259946) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 56 kDa

Observed band size: 50-70 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23933-210

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHF10 also known as PHD finger protein 10 plays a mechanistic role in chromatin remodeling and gene expression. It exhibits a molecular weight of approximately 50 kDa. Researchers have identified PHF10 as a component of the BAF (BRG1-associated factor) chromatin remodeling complex. It is widely expressed in various tissues including the brain where it contributes to neural development and function. Its expression can have tissue-specific variations that reflect its diverse roles in different cellular contexts.
Biological function summary

PHD finger protein 10 participates in the regulation of gene transcription and chromatin architecture. It functions as part of the BAF complex which orchestrates the dynamic remodeling of chromatin to either activate or repress specific genes. This action facilitates cellular processes like differentiation proliferation and response to environmental signals. PHF10 through its interaction with other BAF complex components influences the accessibility of DNA to transcription factors and other regulatory proteins thereby modulating gene expression patterns in cells.

Pathways

PHF10 integrates into the Wnt signaling pathway which is important for cell proliferation and differentiation as well as the Hedgehog signaling pathway important for developmental processes. Within these pathways PHF10 collaborates closely with proteins such as SMARCA4 a core ATPase subunit of the BAF complex. These pathways play fundamental roles in ensuring proper development and maintaining cellular homeostasis where PHF10 activity impacts both normal cellular function and response to environmental changes.

Dysregulation of PHF10 function associates with neurodevelopmental disorders and certain forms of cancer. Altered PHF10 expression or mutations can disrupt normal chromatin remodeling activities leading to improper gene expression profiles. In the context of neurodevelopmental disorders PHF10 misfunction can impact genes critical for nervous system development. In some cancers abnormal interactions between PHF10 and proteins like SMARCC1 another BAF complex member can result in the derangement of gene regulatory networks therefore promoting oncogenic processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information PHF10
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB184951

Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

RabMAb|Recombinant|Advanced Validation

Flow Cytometry (Intracellular) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (AB184951)

  • 0
  • 1
Primary Antibodies

AB303637

Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-TRAP220/MED1 antibody [EPR24656-67] - BSA and Azide free (Detector) (AB303637)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com